Antimicrobial Susceptibility of Canadian Isolates ofHelicobacter pyloriin Northeastern Ontario
| Autor: | Nya L. Fraleigh, Gustavo Ybazeta, Katrina Chapman, Francisco Diaz-Mitoma, Eleonora Altman, Rebecca Letto, Nelson F. Eng |
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| Rok vydání: | 2015 |
| Předmět: |
disk diffusion
Microbiology (medical) Canada genomic DNA antibiotic resistance Resistance Antimicrobial susceptibility Infectious and parasitic diseases RC109-216 minimum inhibitory concentration stomach biopsy Microbiology metronidazole Antibiotic resistance ciprofloxacin epsilometer test medicine antibiotic agent gene mutation gene tetracycline RNA 23S levofloxacin gyra gene amoxicillin Helicobacter pylori biology bacterium isolate bacterial infections and mycoses clarithromycin biology.organism_classification major clinical study antibiotic sensitivity human tissue QR1-502 Infectious Diseases Immunology Northern Ontario Original Article Gastritis medicine.symptom |
| Zdroj: | The Canadian Journal of Infectious Diseases & Medical Microbiology Canadian Journal of Infectious Diseases and Medical Microbiology, Vol 26, Iss 3, Pp 137-144 (2015) |
| ISSN: | 1712-9532 |
| Popis: | Colonization of the stomach with Helicobacter pylori can contribute to the development of gastric ulcers and stomach cancer. The typical treatment course for H pylori infection is a proton pump inhibitor in addition to at least two antibiotics. Increasing resistance to these antibiotics has led to a considerable decline in the eradication rate following treatment over the past several years. The purpose of this study was to assess the rates of resistance to several of the antibiotics commonly used to treat H pylori infection, by investigating a population of patients presenting to a single health care facility in Sudbury, Ontario. Multiple methods were used to assess antibiotic resistance. BACKGROUND: Helicobacter pylori plays a significant role in gastritis and ulcers. It is a carcinogen as defined by the WHO, and infection can result in adenocarcinomas and mucosa-associated lymphoid tissue lymphomas. In Canada, rates of antimicrobial resistance are relatively unknown, with very few studies conducted in the past 15 years. OBJECTIVE: To examine rates of resistance in Sudbury, Ontario, compare antimicrobial susceptibility methods and attempt to determine the molecular basis of antibiotic resistance. METHODS: Patients attending scheduled visits at Health Sciences North (Sudbury, Ontario) provided gastric biopsy samples on a volunteer basis. In total, 20 H pylori isolates were collected, and antimicrobial susceptibility testing (on amoxicillin, tetracycline, metronidazole, ciprofloxacin, levofloxacin and clarithromycin) was conducted using disk diffusion and E-test methods. Subsequently, genomic DNA from these isolates was sequenced to detect mutations associated with antimicrobial resistance. RESULTS: Sixty-five percent of the isolates were found to be resistant to at least one of the listed antibiotics according to E-test. Three isolates were found to be resistant to ≥3 of the above-mentioned antibiotics. Notably, 25% of the isolates were found to be resistant to both metronidazole and clarithromycin, two antibiotics that are normally prescribed as part of first-line regimens in the treatment of H pylori infections in Canada and most of the world. Among the resistant strains, the sequences of 23S ribosomal RNA and gyrA, which are linked to clarithromycin and ciprofloxacin/levofloxacin resistance, respectively, revealed the presence of known point mutations associated with antimicrobial resistance. CONCLUSIONS: In general, resistance to metronidazole, ciprofloxacin/levofloxacin and clarithromycin has increased since the studies in the early 2000s. These results suggest that surveillance programs of H pylori antibiotic resistance may need to be revisited or improved to prevent antimicrobial therapy failure. |
| Databáze: | OpenAIRE |
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