Effect of Ultrafiltration of Pitaya Extract (Stenocereus thurberi) on Its Phytochemical Content, Antioxidant Capacity, and UPLC-DAD-MS Profile
Autor: | José Agustín Tapia-Hernández, Beatriz Montaño-Leyva, Josué Elías Juárez-Onofre, Carlos Gregorio Barreras-Urbina, Daniela Denisse Castro-Enríquez, Elizabeth Carvajal-Millan, Guadalupe Amanda López-Ahumada, Carmen L. Del Toro-Sánchez, Francisco Rodríguez-Félix |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
030309 nutrition & dietetics
DPPH pitaya Ultrafiltration Pharmaceutical Science phytochemical antioxidant capacity High-performance liquid chromatography Analytical Chemistry Stenocereus lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology lcsh:Organic chemistry Drug Discovery Gallic acid Food science Physical and Theoretical Chemistry stenocereus thurberi 0303 health sciences ABTS biology Organic Chemistry 04 agricultural and veterinary sciences biology.organism_classification Stenocereus thurberi uplc-dad-ms 040401 food science Phytochemical chemistry Chemistry (miscellaneous) ultrafiltration Molecular Medicine |
Zdroj: | Molecules, Vol 25, Iss 2, p 281 (2020) Molecules Volume 25 Issue 2 |
ISSN: | 1420-3049 |
Popis: | Stenocereus thurberi is an endemic species in northwestern Mexico. It produces colorful fruits called pitayas that have an edible pulp. They have phytochemical compounds associated with biological activities. Ultrafiltration is a widely used method for the clarification of fruit juices and the recovery of phytochemicals. However, its effect has not been extensively studied in extracts. Therefore, the objective of this work is to study the effect of the ultrafiltration of pitaya extract (Stenocereus thurberi) on its phytochemical content, antioxidant capacity, and identification of phenolic compounds by UPLC-DAD-MS, providing greater knowledge about the pitaya. In this study, two extracts were analyzed, the unclarified extract (UE) and the clarified extract (CE). The antioxidant capacity was higher in the CE with 15.93 ± 0.42 mM TE/g, DPPH and 18.37 ± 0.016 mM TE/g, ABTS. The UPLC-MS analysis indicated the decrease in phenolic compounds in the CE and the presence of gallic acid and resorcinol, compounds that had not been identified in other species of Stenocereus spp. The correlation analysis indicated that all the phytochemicals present in the pitaya contribute significantly to the antioxidant capacity. The ultrafiltration process could be a viable option to improve the biological activity of the natural extracts. |
Databáze: | OpenAIRE |
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