Human CD8+ T Cells Target Multiple Epitopes in Respiratory Syncytial Virus Polymerase
| Autor: | Patrick Günther, Kevin M. Dennehy, Gerhard Jahn, Janet Kerstin Peper, Daniel Burbulla |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Immunology Antigen presentation Human leukocyte antigen Biology CD8-Positive T-Lymphocytes Virus Epitope 03 medical and health sciences Epitopes 0302 clinical medicine Antigen Virology Cell Line Tumor Cytotoxic T cell Humans Protein Isoforms Amino Acid Sequence Antigens Viral Antigen Presentation HLA-A Antigens Viral Vaccines DNA-Directed RNA Polymerases 030104 developmental biology Epitope mapping Respiratory Syncytial Virus Human Vaccines Subunit Molecular Medicine Brief Reports K562 Cells Peptides Immunologic Memory CD8 Epitope Mapping 030215 immunology Protein Binding |
| Popis: | Respiratory syncytial virus (RSV) infection is a serious health problem in young children, immunocompromised patients, and the elderly. The development of novel prevention strategies, such as a vaccine to RSV, is a high priority. One strategy is to design a peptide-based vaccine that activates appropriate CD8(+) T-cell responses. However, this approach is limited by the low number of RSV peptide epitopes defined to date that activate CD8(+) T cells. We aimed to identify peptide epitopes that are presented by common human leukocyte antigen types (HLA-A*01, -A*02, and -B*07). We identify one novel HLA-A*02-restricted and two novel HLA-A*01-restricted peptide epitopes from RSV polymerase. Peptide-HLA multimer staining of specific T cells from healthy donor peripheral blood mononuclear cell, the memory phenotype of such peptide-specific T cells ex vivo, and functional IFNγ responses in short-term stimulation assays suggest that these peptides are recognized during RSV infection. Such peptides are candidates for inclusion into a peptide-based RSV vaccine designed to stimulate defined CD8(+) T-cell responses. |
| Databáze: | OpenAIRE |
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