Evaluating biochemical differences in thyroglobulin from normal and goiter tissues by infrared spectral imaging
| Autor: | Thiago Martini Pereira, Denise Maria Zezell, Benjamin Bird, Max Diem, Luciano Bachmann, Milos Miljkovic |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
endocrine system
Goiter Glycosylation endocrine system diseases Spectrophotometry Infrared medicine.medical_treatment Biochemistry Thyroglobulin Analytical Chemistry chemistry.chemical_compound Electrochemistry medicine Environmental Chemistry Humans Protein secondary structure Spectroscopy chemistry.chemical_classification Triiodothyronine Chemistry Thyroid medicine.disease carbohydrates (lipids) Thyroxine medicine.anatomical_structure lipids (amino acids peptides and proteins) sense organs Glycoprotein Hormone |
| Zdroj: | The Analyst. 145(24) |
| ISSN: | 1364-5528 |
| Popis: | Thyroglobulin is a glycoiodoprotein that is produced by thyroid follicular cells; it is stored in follicles in structures known as colloids. The main function of this protein is to stock the hormones triiodothyronine (T3) and thyroxine (T4) until the body requires them. This study aims to demonstrate that infrared spectral imaging with appropriate multivariate analysis can reveal biochemical changes in this glycoprotein. The results achieved herein point out biochemical differences in the colloid samples obtained from normal and goiter patients including glycosylation and changes in the secondary conformational structure. We have presented the first spectral histopathology-based method to detect biochemical differences in thyroid colloids, such as TG iodination, glycosylation, and changes in the secondary structure in normal and goiter patients. The observed changes in the colloids were mainly due to the alterations in amide I and amide II (secondary conformation of proteins) and there is a correlation with different glycosylation between normal and goiter tissues. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|