Characterization of a major late herpes simplex virus type 1 mRNA
| Autor: | B G Devi, K P Anderson, R H Costa, Edward K. Wagner, Beverley H. Gaylord |
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| Rok vydání: | 1981 |
| Předmět: |
Genes
Viral Transcription Genetic Polyadenylation Immunology DNA Recombinant EcoRI Microbiology Viral Proteins Virology Protein biosynthesis Simplexvirus Coding region RNA Messenger BglII Genetics Messenger RNA Base Sequence biology RNA DNA Restriction Enzymes Molecular biology Protein Biosynthesis Insect Science biology.protein RNA Viral BamHI Research Article |
| Zdroj: | Journal of Virology. 38:483-496 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | A major, late 6-kilobase (6-kb) mRNa mapping in the large unique region of herpes simplex virus type 1 (HSV-1) was characterized by using two recombinant DNA clones, one containing EcoRI fragment G (0.190 to 0.30 map units) in lambda. WES.B (L. Enquist, M. Madden, P. Schiop-Stansly, and G. Vandl Woude, Science 203:541-544, 1979) and one containing HindIII fragment J (0.181 to 0.259 map units) in pBR322. This 6-kb mRNA had its 3' end to the left of 0.231 on the prototypical arrangement of the HSV-1 genome and was transcribed from right to left. It was bounded on both sides by regions containing a large number of distinct mRNA species, and its 3' end was partially colinear with a 1.5-kb mRNA which encoded a 35,000-dalton polypeptide. The 6-kb mRNA encoded a 155,000-dalton polypeptide which was shown to be the only one of this size detectable by hybrid-arrested translation encoded by late polyadenylated polyribosomal RNA. The S1 nuclease mapping experiments indicated that there were no introns in the coding sequence for this mRNA and that its 3' end mapped approximately 800 nucleotides to the left of the BglII site at 0.231, whereas its 5' end extended very close to the BamHI site at 0.266. |
| Databáze: | OpenAIRE |
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