Nerve growth factor contributes to the up-regulation of growth-associated protein 43 and preprotachykinin a messenger RNAs in primary sensory neurons following peripheral inflammation
| Autor: | Piers C. Emson, P.M. Dowd, T A Leslie, Clifford J. Woolf |
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| Rok vydání: | 1995 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Neuropeptide Nerve Tissue Proteins Substance P Biology Rats Sprague-Dawley chemistry.chemical_compound GAP-43 Protein Ganglia Sensory Dorsal root ganglion Tachykinins Internal medicine medicine Animals Nerve Growth Factors RNA Messenger Protein Precursors Gap-43 protein In Situ Hybridization Inflammation Messenger RNA Membrane Glycoproteins General Neuroscience Growth factor Immunohistochemistry Sensory neuron Rats Up-Regulation Endocrinology Nerve growth factor medicine.anatomical_structure chemistry biology.protein |
| Zdroj: | Neuroscience. 67:753-761 |
| ISSN: | 0306-4522 |
| DOI: | 10.1016/0306-4522(95)00101-n |
| Popis: | Peripheral inflammation induced in adult rats by an intraplantar injection of complete Freund's adjuvant results in a rapid (6h) increase in the expression of the messenger RNAs for the neuronal growth-associated protein 43 and for preprotachykinin A, the precursor for substance P, in dorsal root ganglion sensory neurons innervating the inflamed area. This increase peaks at 48 h and then declines by five days. The changes are present in the dorsal root ganglion cells innervating the inflamed skin (lumbar 4 or 5) but no elevation was found in the third lumbar dorsal root ganglion which innervates neighbouring non-inflamed skin. The increased growth-associated protein 43 messenger RNA in the dorsal root ganglion is followed by a marked increase in growth-associated protein 43-like immunoreactive fibres in the epidermis of the inflamed skin. Systemic administration of neutralizing anti-nerve growth factor antibodies immediately prior to the inflammation prevents the increase in growth-associated protein 43 and preprotachykinin A messenger RNAs in the sensory neurons. A subcutaneous injection of nerve growth factor (200 ng) into the hindpaw elevates preprotachykinin A but not growth-associated protein 43 messenger RNA in the fourth lumbar dorsal root ganglion 48 h post-injection and this could be prevented by co-administration of the anti-nerve growth factor serum. The production of nerve growth factor in inflamed target tissues leads to alterations in the phenotype of responsive adult primary sensory neurons which include a change in the levels of a growth-related protein and a peptide neuromodulator. This raises the possibility that inflammation and its associated hypersensitivity may involve both an alteration in the growth status of sensory neurons and in their neuropeptide content. |
| Databáze: | OpenAIRE |
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