A 3D printed nano bone matrix for characterization of breast cancer cell and osteoblast interactions
| Autor: | Lijie Grace Zhang, Nathan J. Castro, Robert I. Glazer, Robert McGrane, Xuan Zhou, Wei Zhu, Haitao Cui, Benchaa Boualam |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Materials science Mechanical Engineering Spheroid Bone metastasis Bioengineering Osteoblast General Chemistry medicine.disease In vitro 03 medical and health sciences 030104 developmental biology Breast cancer medicine.anatomical_structure Mechanics of Materials Tumor progression Cell culture medicine Cancer research General Materials Science Secretion Electrical and Electronic Engineering |
| Zdroj: | Nanotechnology. 27:315103 |
| ISSN: | 1361-6528 0957-4484 |
| DOI: | 10.1088/0957-4484/27/31/315103 |
| Popis: | Bone metastasis is one of the most prevalent complications of late-stage breast cancer, in which the native bone matrix components, including osteoblasts, are intimately involved in tumor progression. The development of a successful in vitro model would greatly facilitate understanding the underlying mechanism of breast cancer bone invasion as well as provide a tool for effective discovery of novel therapeutic strategies. In the current study, we fabricated a series of in vitro bone matrices composed of a polyethylene glycol hydrogel and nanocrystalline hydroxyapatite of varying concentrations to mimic the native bone microenvironment for the investigation of breast cancer bone metastasis. A stereolithography-based three-dimensional (3D) printer was used to fabricate the bone matrices with precisely controlled architecture. The interaction between breast cancer cells and osteoblasts was investigated in the optimized bone matrix. Using a Transwell® system to separate the two cell lines, breast cancer cells inhibited osteoblast proliferation, while osteoblasts stimulated breast cancer cell growth, whereas, both cell lines increased IL-8 secretion. Breast cancer cells co-cultured with osteoblasts within the 3D bone matrix formed multi-cellular spheroids in comparison to two-dimensional monolayers. These findings validate the use of our 3D printed bone matrices as an in vitro metastasis model, and highlights their potential for investigating breast cancer bone metastasis. |
| Databáze: | OpenAIRE |
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