Lytic polysaccharide monooxygenase from Aspergillus fumigatus can improve enzymatic cocktail activity during sugarcane bagasse hydrolysis
| Autor: | Luis Eduardo Gerolamo, Aline Vianna Bernardi, Lucas Matheus Soares Pereira, Sérgio Akira Uyemura, Paula Fagundes de Gouvêa, Taisa Magnani Dinamarco |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Cellulase Polysaccharide 01 natural sciences Biochemistry Mixed Function Oxygenases Aspergillus fumigatus Fungal Proteins 03 medical and health sciences Polysaccharides Structural Biology 010608 biotechnology Protein purification Escherichia coli ASPERGILLUS Biomass Cellulose 030304 developmental biology chemistry.chemical_classification 0303 health sciences Ethanol biology Molecular mass Hydrolysis General Medicine Monooxygenase biology.organism_classification Recombinant Proteins Saccharum Enzyme chemistry biology.protein Oxidation-Reduction Peroxidase |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Background: Lytic Polysaccharide Monooxygenases (LPMOs) are auxiliary accessory enzymes that act synergistically with cellulases and which are increasingly being used in secondgeneration bioethanol production from biomasses. Several LPMOs have been identified in various filamentous fungi, including Aspergillus fumigatus. However, many LPMOs have not been characterized yet. Objective: To report the role of uncharacterized A. fumigatus AfAA9_B LPMO. Methods: qRT-PCR analysis was employed to analyze the LPMO gene expression profile in different carbon sources. The gene encoding an AfAA9_B (Afu4g07850) was cloned into the vector pET- 28a(+), expressed in the E. coli strain RosettaTM (DE3) pLysS, and purified by a Ni2+-nitrilotriacetic (Ni-NTA) agarose resin. To evaluate the specific LPMO activity, the purified protein peroxidase activity was assessed. The auxiliary LPMO activity was investigated by the synergistic activity in Celluclast 1.5L enzymatic cocktail. Results: LPMO was highly induced in complex biomass like sugarcane bagasse (SEB), Avicel® PH-101, and CM-cellulose. The LPMO gene encoded a protein comprising 250 amino acids, without a CBM domain. After protein purification, the AfAA9_B molecular mass estimated by SDSPAGE was 35 kDa. The purified protein specific peroxidase activity was 8.33 ± 1.9 U g-1. Upon addition to Celluclast 1.5L, Avicel® PH-101 and SEB hydrolysis increased by 18% and 22%, respectively. Conclusion: A. fumigatus LPMO is a promising candidate to enhance the currently available enzymatic cocktail and can therefore be used in second-generation ethanol production. |
| Databáze: | OpenAIRE |
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