Self-inhibition of steroid secretion by amphibian adrenocortical cells is not mediated through glucocorticoid receptors
| Autor: | P. Netchitailo, Hubert Vaudry, F. Leboulenger, A. Larcher, M. Feuilloley, D. Philibert |
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| Rok vydání: | 1991 |
| Předmět: |
Agonist
Male medicine.medical_specialty medicine.drug_class Biology In Vitro Techniques chemistry.chemical_compound Endocrinology Glucocorticoid receptor Receptors Glucocorticoid Corticosterone Internal medicine medicine Animals Molecular Biology Chromatography High Pressure Liquid Rana ridibunda Aldosterone Adrenal cortex medicine.anatomical_structure chemistry Pregnenolone Adrenal Cortex Glucocorticoid Endocrine gland medicine.drug |
| Zdroj: | Journal of molecular endocrinology. 6(3) |
| ISSN: | 0952-5041 |
| Popis: | To investigate a possible direct action of glucocorticoids on adrenal steroidogenesis, the effect of corticosterone on the conversion of pregnenolone into various metabolites by frog adrenal tissue was examined. Frog interrenal slices were incubated with [3H]pregnenolone (1 mCi/ml) and the various labelled metabolites analysed by reverse-phase high-performance liquid chromatography. With the methanol gradient used, five identified steroids were resolved: progesterone, 11-deoxycorticosterone, corticosterone, 18-hydroxycorticosterone and aldosterone. Corticosterone (10 μg/ml) induced a 45–80% decrease in all steroids synthesized from [3H]pregnenolone. In contrast, the glucocorticoid agonist dexamethasone did not reduce the rate of conversion of pregnenolone into its metabolites. In addition, the inhibitory effect of corticosterone was not reversed by the specific glucocorticoid antagonist RU 43044. These results show that corticosterone exerts a direct inhibitory effect on adrenal steroid secretion. In addition, our data indicate that the ultra-short regulation induced by corticosterone is not mediated through glucocorticoid receptors. |
| Databáze: | OpenAIRE |
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