Adipose tissue-derived stromal cells acquire endothelial-like features upon reprogramming with SOX18
| Autor: | S.L. Ragghoe, Anton J.G. Horrevoets, Ester M. Weijers, Elisa Meinster, Nanne J. Paauw, Ruud D. Fontijn, Tessa D. Nauta, M.A. van den Broek, B.A. Naaijkens, Julie Favre, H.W.M. Niessen, Pieter Koolwijk |
|---|---|
| Přispěvatelé: | Molecular cell biology and Immunology, Pathology, Physiology, Cardio-thoracic surgery, ICaR - Ischemia and repair |
| Jazyk: | angličtina |
| Předmět: |
Vascular Endothelial Growth Factor A
Stromal cell Cellular differentiation Genetic Vectors Adipose tissue Biology Microtubules Regenerative medicine Cell Movement Human Umbilical Vein Endothelial Cells SOXF Transcription Factors Humans lcsh:QH301-705.5 Cells Cultured Medicine(all) Chemotaxis Endothelial Cells Cell Differentiation Cell Biology General Medicine Cellular Reprogramming Cell biology Vascular endothelial growth factor A Adipose Tissue lcsh:Biology (General) Matrix Metalloproteinase 7 Immunology Ectopic expression Stromal Cells Shear Strength Reprogramming Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 13, Iss 3, Pp 367-378 (2014) Stem Cell Research, 13(3 Pt A), 367-378. Elsevier Fontijn, R D, Favre, J, Naaijkens, B A, Meinster, E, Paauw, N J, Ragghoe, S L, Nauta, T D, van den Broek, M A, Weijers, E M, Niessen, H W M, Koolwijk, P & Horrevoets, A J G 2014, ' Adipose tissue-derived stromal cells acquire endothelial-like features upon reprogramming with SOX18 ', Stem Cell Research, vol. 13, no. 3 Pt A, pp. 367-378 . https://doi.org/10.1016/j.scr.2014.09.004 |
| ISSN: | 1873-5061 |
| DOI: | 10.1016/j.scr.2014.09.004 |
| Popis: | Adipose tissue-derived stromal cells (ASC) form a rich source of autologous cells for use in regenerative medicine. In vitro induction of an endothelial phenotype may improve performance of ASCs in cardiovascular repair. Here, we report on an in vitro strategy using direct reprogramming of ASCs by means of ectopic expression of the endothelial-specific transcription factor SRY (sex determining region Y)-box18 (SOX18). SOX18 induces ASCs to express a set of genes involved in vascular patterning: MMP7, KDR, EFNB2, SEMA3G and CXCR4. Accordingly, SOX18 transduced ASCs reorganize under conditions of shear stress, display VEGF-induced chemotaxis and form tubular structures in 3D matrices in an MMP7-dependent manner. These in vitro findings provide insight into molecular and cellular processes downstream of SOX18 and show that reprogramming using SOX18 is sufficient to induce several endothelial-like features in ASCs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|