Calorie restriction inhibits ovarian follicle development and follicle loss through activating SIRT1 signaling in mice
| Autor: | Xing-Mei Zhang, Li-Li Luo, Wei-Juan Liu, Na Wang, Xiao-Ling Zhou, Yu-Cai Fu |
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| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
endocrine system diseases Calorie restriction Regulator SIRT1 signaling Mice Follicle SIRT1 activator Ovarian Follicle Sirtuin 1 Internal medicine Animals Sirtuins Medicine NRF1 Ovarian follicle Caloric Restriction chemistry.chemical_classification biology Nuclear Respiratory Factor 1 business.industry Research Forkhead Box Protein O3 Forkhead Transcription Factors General Medicine Mice Inbred C57BL Enzyme Endocrinology medicine.anatomical_structure chemistry Ovarian development biology.protein Female Tumor Suppressor Protein p53 Signal transduction business hormones hormone substitutes and hormone antagonists Signal Transduction |
| Zdroj: | European Journal of Medical Research |
| ISSN: | 2047-783X |
| Popis: | Background Silent information regulator 2 related enzyme 1 (SIRT1) is one of the key factors in the mechanism of calorie restriction (CR) extending lifespan of animals. The aim of the study is to investigate if CR prolongs ovarian lifespan in mice through activating SIRT1 signaling. Methods In the present study, 21 female C57BL/6 mice were divided into three groups: the control (n = 7), CR (n = 7), and SRT1720 (n = 7) groups. After the 26-week treatment, the number of ovarian follicles at each stage was counted, and Western blot was performed. Results The number of surviving follicles in ovaries of the SRT1720 group was less than that of the CR group but more than that of the normal control (NC) group. The number of atretic follicles in the ovaries of the SRT1720 group was similar to that of the CR group but less than that of the NC group. The number of primordial follicles in the ovaries of the SRT1720 group was less than that of the CR group but more than that of the NC group. The numbers of primary follicles, secondary follicles, antral follicles, and corpora lutea in the SRT1720 group were similar to those in the CR group. Western blot analysis showed that the expression of SIRT1, SIRT6, FOXO3a, and NRF1 proteins was upregulated, and p53 was downregulated in both the CR group and the SRT1720 group compared to the control group. Conclusions Our results indicate that CR inhibits the activation of primordial follicles and development of follicles at different stages, thus preserving the reserve of follicle pool (at least partly) through activating SIRT1 signaling. |
| Databáze: | OpenAIRE |
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