Replication of Neurotropic Influenza Virus in Organotypic Cultures of Embryonic Mouse Hypothalamus
| Autor: | Konrad C. Hsu, Abner Wolf, Helena H. Benitez, Philip E. Duffy, Eugenia T. Gamboa, Naohiko Takahata, Donald H. Harter |
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| Rok vydání: | 1974 |
| Předmět: |
Cytoplasm
Virus Cultivation Nucleolus viruses Neurotropism Orthomyxoviridae Hypothalamus Fluorescent Antibody Technique Virus Replication Virus Pathology and Forensic Medicine Mice Cellular and Molecular Neuroscience Cytopathogenic Effect Viral Culture Techniques medicine Animals Antigens Viral Cell Nucleus biology General Medicine biology.organism_classification Embryonic stem cell Virology Microscopy Electron Cytolysis medicine.anatomical_structure Neurology Organ Specificity Neuroglia Neurology (clinical) Intracellular |
| Zdroj: | Journal of Neuropathology & Experimental Neurology. 33:571-581 |
| ISSN: | 1554-6578 0022-3069 |
| DOI: | 10.1097/00005072-197410000-00001 |
| Popis: | Neurotropic influenza virus (WS-N strain) causes cytolytic changes in organotypic neural cultures derived from embryonic mouse hypothalamus. Initially, nerve cells develop enlarged, refractile nucleoli. In later stages, both nerve and glial cells undergo necrotic changes. These alterations are accompanied by synthesis of progeny virus and formation of intracellular viral antigen. Electron microscopic observations of infected cultures reveal virus particles with the morphology and dimensions of influenza virions. Organotypic neural cultures may prove useful in studies of influenza virus neurotropism. |
| Databáze: | OpenAIRE |
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