Leptin Distribution in Rat Foetal and Extraembryonic Tissues in Late Gestation: A Physiological View of Amniotic Fluid Leptin

Autor: Andreu Palou, Catalina Picó, Zhi Xin Yau-Qiu, Ana M. Rodríguez
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
0301 basic medicine
Leptin
Amniotic fluid
embarazo
distribución tisular
intercambio materno-fetal
humanos
Extraembryonic Membranes
gastric content
ratas
Umbilical cord
Fetal Development
0302 clinical medicine
Pregnancy
Tissue Distribution
Maternal-Fetal Exchange
late gestation
Nutrition and Dietetics
Stomach
digestive
oral
and skin physiology
edad gestacional
medicine.anatomical_structure
mucosa gástrica
Gestation
Immunohistochemistry
Female
leptina
lcsh:Nutrition. Foods and food supply
hormones
hormone substitutes
and hormone antagonists
medicine.medical_specialty
feto
placenta
Offspring
030209 endocrinology & metabolism
lcsh:TX341-641
Gestational Age
Biology
Article
foetal stomach
03 medical and health sciences
Fetus
membranas extraembrionarias
Placenta
Internal medicine
desarrollo fetal
metabolic programming
medicine
Humans
Animals
Rats
Wistar
líquido amniótico
amniotic fluid
Rats
030104 developmental biology
Endocrinology
Gastric Mucosa
animales
Food Science
Zdroj: Nutrients, Vol 12, Iss 2542, p 2542 (2020)
Nutrients
Volume 12
Issue 9
DOI: 10.3390/nu12092542
Popis: Prenatal leptin is key to regulating foetal growth and early metabolic programming. The presence of intact leptin in rat foetal (at late gestation) and neonatal (immediately after birth) stomach content and mucosa has been previously described, suggesting that it may act as a regulatory nutrient for the neonate rats, be internalised by the stomach, and play a physiological role early in life, which requires to be further investigated, including its origin. We aimed to study the ontogeny of the presence of leptin in the foetal stomach and key extraembryonic tissues in rats at late gestation (days 18-21). Leptin concentration was determined by enzyme-linked immunosorbent assay, and placental leptin immunolocalisation was analysed by immunohistochemistry. Leptin showed a sudden appearance in the amniotic fluid (AF) at day 20 of gestation, gastric content (swallowed AF), stomach, and umbilical cord, significantly increasing at day 21. Leptin levels in these fluids and tissues were positively correlated. In the placenta, leptin was detectable at all the studied days, but its localisation changed from widespread throughout the placenta at day 18 to well-defined in the labyrinth zone from day 19 onwards. The results support a possible internalisation of AF leptin by the immature stomach of near-term foetuses and suggest that changes in placental leptin localisation might help to explain the sudden appearance of leptin in AF at gestational day 20, with potential physiological significance regarding short-term feeding control and metabolic programming in the developing offspring.
This research was supported by the Spanish Government (grants AGL2015-67019-P (MINECO/FEDER, UE) and PGC2018-097436-B-I00 (MCIU/AEI/FEDER, UE)), and the Instituto de Salud Carlos III, Centro de Investigacion Biomedica en Red Fisiopatologia de la Obesidad y Nutricion, CIBERobn. Z.X.Y.-Q. was granted a PhD fellowship funded by the Spanish Government, grant number FPU014/0386.
Databáze: OpenAIRE
Externí odkaz: