Precision of readout at the hunchback gene
| Autor: | Mathieu Coppey, Cécile Fradin, Teresa Ferraro, Jonathan Desponds, Nathalie Dostatni, Aleksandra M. Walczak, Tanguy Lucas, Aurelien Guillou, Huy Tran, Perez Romero C |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Genetics
0303 health sciences animal structures Computational biology Biology Imaging data Transcription initiation 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Transcription (biology) medicine Nucleus Gene Developmental biology 030217 neurology & neurosurgery 030304 developmental biology |
| DOI: | 10.1101/063784 |
| Popis: | The simultaneous expression of the hunchback gene in the multiple nuclei of the developing fly embryo gives us a unique opportunity to study how transcription is regulated in functional organisms. A recently developed MS2-MCP technique for imaging transcription in living Drosophila embryos allows us to quantify the dynamics of the developmental transcription process. The initial measurement of the morphogens by the hunchback promoter takes place during very short cell cycles, not only giving each nucleus little time for a precise readout, but also resulting in short time traces. Additionally, the relationship between the measured signal and the promoter state depends on the molecular design of the reporting probe. We develop an analysis approach based on tailor made autocorrelation functions that overcomes the short trace problems and quantifies the dynamics of transcription initiation. Based on life imaging data, we identify signatures of bursty transcription initiation from the hunchback promoter. We show that the precision of the expression of the hunchback gene to measure its position along the anterior-posterior axis is low both at the boundary and in the anterior even at cycle 13, suggesting additional post-translational averaging mechanisms to provide the precision observed in fixed material. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|