The E1 α and β Subunits of the Pyruvate Dehydrogenase Complex are M2‘d’ and M2‘e’ Autoantigens in Primary Biliary Cirrhosis
| Autor: | Oliver F. W. James, Dorothy Fittes, Ian B. Turner, Stephen J. Yeaman, Shelley P. M. Fussey, Margaret F. Bassendine |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
medicine.medical_specialty
Biliary cirrhosis Immunoblotting Alpha (ethology) Pyruvate Dehydrogenase Complex Biology Autoantigens Primary biliary cirrhosis Internal medicine medicine Animals Humans Beta (finance) Liver Cirrhosis Biliary Liver Diseases General Medicine medicine.disease Pyruvate dehydrogenase complex Molecular biology Mitochondria Endocrinology biology.protein Cattle Electrophoresis Polyacrylamide Gel Female Antibody Oxoglutarate dehydrogenase complex Branched-chain alpha-keto acid dehydrogenase complex |
| Zdroj: | Clinical Science. 77:365-368 |
| ISSN: | 1470-8736 0143-5221 |
| DOI: | 10.1042/cs0770365 |
| Popis: | 1. Sera from 76 patients with primary biliary cirrhosis (PBC) and 66 control subjects (53 with chronic liver disease and 13 healthy normal women) were immunoblotted against purified E1 component of bovine pyruvate dehydrogenase complex (PDC) and bacterial PDC. 2. Thirty-one out of seventy-six (41%) sera from PBC patients showed a positive response to bovine E1α, and five of these 31 (7% of total) reacted with bovine E1β. None of the control sera reacted with bovine E1 α or β. 3. None of the PBC sera that recognized bovine E1 subunits reacted with bacterial PDC E1. 4. In the PBC patients there was no correlation between presence of antibodies to E1α and β subunits and histological stage of the disease. 5. Our data demonstrate that the E1α and β components of mammalian PDC are the M2‘d’ and ‘e’ mitochondrial autoantigens, respectively. |
| Databáze: | OpenAIRE |
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