A Thiol Protease and an Anionic Peroxidase Are Induced by Lowering Cytokinins during Callus Growth in Petunia

Autor: Colette Tournaire, S. Kushnir, G. Bauw, B. T. de la Serve, Jean-Pierre Renaudin, Dirk Inzé
Přispěvatelé: Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
Rok vydání: 1996
Předmět:
0106 biological sciences
Cytokinins
DNA
Complementary
Physiology
Molecular Sequence Data
Plant Development
Organogenesis
Plant Science
01 natural sciences
Petunia
Gene Expression Regulation
Enzymologic
[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics
03 medical and health sciences
Tissue culture
chemistry.chemical_compound
Gene Expression Regulation
Plant
Arabidopsis
Genetics
Amino Acid Sequence
RNA
Messenger
Cloning
Molecular
ComputingMilieux_MISCELLANEOUS
Peroxidase
Plant Proteins
030304 developmental biology
0303 health sciences
Base Sequence
Sequence Homology
Amino Acid
biology
fungi
food and beverages
Plants
biology.organism_classification
Cysteine Endopeptidases
Biochemistry
chemistry
Enzyme Induction
Callus
Cytokinin
biology.protein
Solanaceae
Research Article
010606 plant biology & botany
Zdroj: Plant Physiology
Plant Physiology, American Society of Plant Biologists, 1996, 111, pp.159-168. ⟨10.1104/pp.111.1.159⟩
ISSN: 1532-2548
0032-0889
DOI: 10.1104/pp.111.1.159
Popis: We previously identified a group of proteins that increase early in Petunia hybrida calli subcultured on a low-cytokinin medium, unlike the calli subcultured on a high-cytokinin medium. The calli on the low-cytokinin medium do not regenerate (J.-P. Renaudin, C. Tournaire, B. Teyssendier de la Serve [1991] Physiol Plant 82: 48–56). Two of these proteins, P21 and P17, have been identified by peptide sequencing and cloned. P21 is highly homologous to a group of thiol proteases, including barley aleurain, rice oryzain [gamma], Arabidopsis SAG2, and mammalian cathepsin H. P17 is highly homologous to a group of anionic peroxidases from potato and tomato. A study of their expression in two P. hybrida lines, PC6 and St40, which differ in their ability to regenerate, showed that the genes for P21 and P17 are differentially expressed depending on the type and the age of the organ, with the highest expression in senescing leaves and in aged calli. The data are in favor of these genes being associated with an early step of senescence, which may be due, in part, to a reduction in total cytokinin. The two Petunia lines are, thus, functionally different concerning the action of cytokinin in two developmental phenomena: in vitro organogenesis and senescence.
Databáze: OpenAIRE
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