The application of Ussing chambers for determining the impact of microbes and probiotics on intestinal ion transport
| Autor: | Niall P. Hyland, Kevin W. Lomasney |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Pharmacology
Ion Transport Ussing chamber Intestinal Secretions Physiology Gastrointestinal Diseases Probiotics Organic disorders Membrane Transport Proteins General Medicine Biology Intestinal absorption Microbiology Intestines Intestinal Absorption Physiology (medical) Host-Pathogen Interactions Ion secretion Animals Diffusion Chambers Culture Humans Intestinal Mucosa Ion transporter Signal Transduction |
| Zdroj: | Canadian journal of physiology and pharmacology. 91(9) |
| ISSN: | 1205-7541 |
| Popis: | Host–microbe interactions have gained considerable attention in recent years with regards to their role in various organic disorders and diseases. In particular, research efforts have focused on the intestinal microbiota, where the largest and most diverse populations not only co-exist with the host, but also directly influence the state and function of the gastrointestinal (GI) tract. Moreover, both human and animal studies alike are now beginning to show a positive influence of probiotic bacteria on GI disorders associated with diarrhoea or constipation. Diarrheagenic GI diseases, such as those caused by Vibreo cholera or enterpathogenic Eschericia coli, have well-characterised interactions with the host that explain much of the observed symptoms, in particular severe diarrhoea. However, the mechanisms of action of nonpathogenic bacteria or probiotics on host physiology are less clearly understood. In the context of defining the mechanisms of action of probiotics in vitro, the Ussing chamber has proven to be a particularly useful tool. Here, we will present data from several studies that have defined molecular targets for microbes and putative probiotics in the regulation of intestinal secretory and absorptive function, and we will discuss these in the context of their application in pathogen- or inflammation-induced alterations in intestinal ion transport. |
| Databáze: | OpenAIRE |
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