SULF2 promotes tumorigenesis and inhibits apoptosis of cervical cancer cells through the ERK/AKT signaling pathway

Autor: Tao Jiang, Zhao-Hui Chen, Zhe Chen, Dan Tan
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
MAPK/ERK pathway
Medicine (General)
Physiology
Proliferation
Uterine Cervical Neoplasms
Apoptosis
SULF2
medicine.disease_cause
Biochemistry
HeLa
Mice
0302 clinical medicine
Biology (General)
General Pharmacology
Toxicology and Pharmaceutics
biology
Chemistry
General Neuroscience
General Medicine
Middle Aged
Immunohistochemistry
Gene Expression Regulation
Neoplastic
ERK/AKT
030220 oncology & carcinogenesis
Female
Sulfatases
Signal transduction
Signal Transduction
Research Article
Adult
MAP Kinase Signaling System
QH301-705.5
Immunology
Biophysics
Mice
Nude
Ocean Engineering
Real-Time Polymerase Chain Reaction
03 medical and health sciences
R5-920
Downregulation and upregulation
Cell Line
Tumor
medicine
Animals
Humans
Protein kinase B
Aged
Neoplasm Staging
Akt/PKB signaling pathway
Cell Biology
biology.organism_classification
030104 developmental biology
Case-Control Studies
Cancer research
Cervical cancer
Carcinogenesis
Proto-Oncogene Proteins c-akt
HeLa Cells
Zdroj: Brazilian Journal of Medical and Biological Research, Volume: 53, Issue: 2, Article number: e8901, Published: 07 FEB 2020
Brazilian Journal of Medical and Biological Research v.53 n.2 2020
Brazilian Journal of Medical and Biological Research
Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
Brazilian Journal of Medical and Biological Research, Vol 53, Iss 2 (2020)
Popis: The objective of this study was to explore the role of the SULF2-mediated ERK/AKT signaling pathway in cervical cancer. SULF2 expression was detected in tumor tissues and tumor-adjacent normal tissues from cervical cancer patients. HeLa cells were divided into six groups: control group, NC group, SULF2 siRNA group, SULF2 group, SULF2 + LY294002 group, and SULF2 + U0125 group. In each group, HeLa cells received the corresponding treatment, followed by measurement of the cellular biological characteristics and expression of the ERK/AKT signaling pathway. We also confirmed the effect of SULF2 in vivo using a xenograft model in nude mice. SULF2 was upregulated in cervical cancer tissues, which was specifically associated with the clinical stage, histological differentiation, and lymphatic metastasis. Compared to the control group, the SULF2 siRNA group displayed decreased expression of SULF2, concomitant with reduced proliferation, migration, and invasion, but there was an increase in the apoptosis rate of HeLa cells, as well as downregulation of the p-Akt/Akt, p-ERK/ERK, and Bax/Bcl-2 ratios and cyclin D1. Additionally, tumor growth was significantly inhibited in the xenograft model of nude mice. The results in the SULF2 group were quite the opposite in which SULF2 facilitated the growth of cervical cancer cells, which was reversed by LY294002 or U0126. SULF2 is highly expressed in cervical cancer, and thus, downregulation of SULF2 can inhibit the ERK1/2 and AKT signaling pathways to suppress the proliferation, invasion, and migration of cervical cancer cells while facilitating apoptosis.
Databáze: OpenAIRE
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