Intraocular Neurografts as a Model for Studying of Organization of Synaptic Connections in a Denervated Brain Area
| Autor: | Z. N. Zhuravleva, S. S. Khutsyan, G. I. Zhuravlev |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Septal Region Male Transplantation Heterotopic Presynaptic Terminals Cell Communication Biology Synaptic Transmission General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Postsynaptic potential Anterior Eye Segment medicine Animals Transplantation Homologous Rats Wistar Neurons Nervous tissue Significant difference General Medicine Embryo Mammalian Rats 030104 developmental biology medicine.anatomical_structure Astrocytes Tissue Transplantation Septum of Brain Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Bulletin of experimental biology and medicine. 166(6) |
| ISSN: | 1573-8221 |
| Popis: | Intraocular neurografts of the septal region of rats were used as the model of deafferentiated brain area where the lack of adequate innervation is compensated for own interneuronal connections. Septum anlage from the brain of a 17-day fetus served as the donor material. The grafts developing in the anterior eye chamber over 3 months represented well-differentiated samples of the nervous tissue. A comparative morphometric study of the tripartite organization of synapses in the grafts and in the septum in situ was conducted. In the grafts, the mean volume and perimeter of synaptic terminals were below the normal. At the same time, postsynaptic densities did not differ from the control. A significant difference was found in the degree of surrounding of presynaptic terminals by astrocytic processes: in the grafts this parameter was higher by 1.8 times. Our results attest to an important role of perisynaptic glia in the formation of functionally active synaptic contacts with unusual neuronal targets. |
| Databáze: | OpenAIRE |
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