A sensitive method for the quantitation of the peptide-based glucagon-like peptide-1 receptor agonist liraglutide in plasma using microfluidics chromatography tandem MS
| Autor: | Christopher L Holliman, Heather Eng, Amit S. Kalgutkar, Amanda King-Ahmad, Mark Niosi |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
Agonist Bioanalysis medicine.drug_class Clinical Biochemistry 030209 endocrinology & metabolism Peptide Sensitivity and Specificity 01 natural sciences Glucagon-Like Peptide-1 Receptor Analytical Chemistry 03 medical and health sciences 0302 clinical medicine Pharmacokinetics Tandem Mass Spectrometry Liquid chromatography–mass spectrometry medicine Animals Humans Hypoglycemic Agents General Pharmacology Toxicology and Pharmaceutics Glucagon-like peptide 1 receptor chemistry.chemical_classification Chromatography Liraglutide Chemistry 010401 analytical chemistry General Medicine Microfluidic Analytical Techniques 0104 chemical sciences Macaca fascicularis Medical Laboratory Technology Pharmacodynamics Administration Intravenous Terfenadine Chromatography Liquid medicine.drug |
| Zdroj: | Bioanalysis. 10:357-368 |
| ISSN: | 1757-6199 1757-6180 |
| DOI: | 10.4155/bio-2017-0239 |
| Popis: | Aim: An LC–MS/MS assay for the quantitation of liraglutide, a peptide-based injectable glucagon-like peptide-1 receptor agonist, has been developed as a convenient alternative to the enzyme-linked immunosorbent assay, and used to characterize liraglutide pharmacokinetics in cynomolgus monkeys. Results: Assay calibration curves exhibited a linear dynamic range of 10–5000 ng/ml and correlation coefficient ≥0.98. Following a 30 μg/kg intravenous dose, liraglutide demonstrated low plasma clearance and distribution volume, which led to a terminal half-life of 6.59 h in monkeys. Conclusion: The dynamic range of our LC–MS/MS assay provides sufficient coverage of the average efficacious liraglutide concentrations in human plasma, and can be used for pharmacokinetics/pharmacodynamics studies in animals and potentially in humans. |
| Databáze: | OpenAIRE |
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