Iso-seq analysis and functional annotation of the Santa Fe cave crayfish (Procambarus erythrops) transcriptome
| Autor: | James D. Austin, Celine M. Carneiro, Paul E. Moler, Jerald D. Noble, Adele Pietras |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0303 health sciences Procambarus erythrops Sequence Analysis RNA De novo transcriptome assembly Procambarus RNA-Seq Astacoidea Aquatic Science Biology Crayfish biology.organism_classification 010603 evolutionary biology 01 natural sciences Crustacean Transcriptome 03 medical and health sciences Genus Evolutionary biology Genetics Florida Animals 030304 developmental biology |
| Zdroj: | Marine genomics. 58 |
| ISSN: | 1876-7478 |
| Popis: | The genus Procambarus represents a diverse genus of freshwater crayfish that includes epigean species, stygobitic species, and at least one parthenogenic species. Despite its evolutionary, ecological, and economic importance, most genomic and transcriptomic resources for this genus are limited to a couple of model species. We sequenced the transcriptome of a non-model species, P. erythrops, a geographically restricted stygobitic species from Florida. RNA isolated from gill, muscle and eye tissue was pooled to create a de novo transcriptome assembly using Single Molecule Real-Time sequencing (PacBio), resulting in 19,442 full-length isoforms. The assembly has been deposited in the NCBI (BioProject PRJNA657230). These data will make an important contribution to the comparative study of transcriptome evolution in crayfish and crustaceans. |
| Databáze: | OpenAIRE |
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