Construction of protein-modified TiO2 nanoparticles for use with ultrasound irradiation in a novel cell injuring method
| Autor: | Keiko Takaki, Yusuke Miyachi, Nobuaki Shimizu, Kazuaki Ninomiya, Naonori Shibata, Chiaki Ogino, Shun'ichi Kuroda, Ryosuke Sasai |
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| Rok vydání: | 2010 |
| Předmět: |
Stereochemistry
medicine.medical_treatment Clinical Biochemistry Pharmaceutical Science chemistry.chemical_element Nanoparticle Photodynamic therapy Biochemistry chemistry.chemical_compound Cell Line Tumor Drug Discovery medicine Humans Ultrasonics Surface plasmon resonance Molecular Biology Titanium business.industry Organic Chemistry Ultrasound technology industry and agriculture Proteins chemistry Titanium dioxide Cancer cell Nanoparticles Molecular Medicine Drug carrier business Nuclear chemistry |
| Zdroj: | Bioorganic & Medicinal Chemistry Letters. 20:5320-5325 |
| ISSN: | 0960-894X |
| DOI: | 10.1016/j.bmcl.2010.06.124 |
| Popis: | Recently, our group discovered an alternative titanium dioxide (TiO(2)) activation method that uses ultrasound irradiation (US/TiO(2)) instead of ultraviolet irradiation. The pre-S1/S2 protein from hepatitis B virus, which recognizes liver cells, was immobilized to the surface of TiO(2) nanoparticles using an amino-coupling method. The ability of the protein-modified TiO(2) nanoparticles to recognize liver cells was confirmed by surface plasmon resonance analysis and immuno-staining analyses. After uptake of TiO(2) nanoparticles by HepG2 cancer cells, the cells were injured using this US/TiO(2) method; significant cell injury was observed at an ultrasound irradiation intensity of 0.4 W/cm(2). Together with these results, this strategy could be applied to new cell injuring systems that use ultrasound irradiation in place of photodynamic therapy in the near future. |
| Databáze: | OpenAIRE |
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