Structural characterization of an all-aminosugar-containing capsular polysaccharide from Colwellia psychrerythraea 34H
| Autor: | Ermenegilda Parrilli, Angela Casillo, Maria Michela Corsaro, Jonas Ståhle, Daniel E. Mitchell, Rosa Lanzetta, Michelangelo Parrilli, Filomena Sannino, Göran Widmalm, Gennaro Marino, Maria Luisa Tutino, Giuseppina Pieretti, Matthew I. Gibson |
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| Přispěvatelé: | Casillo, Angela, Ståhle, Jona, Parrilli, Ermenegilda, Sannino, Filomena, Mitchell, Daniel E., Pieretti, Giuseppina, Gibson, Matthew I., Marino, Gennaro, Lanzetta, Rosa, Parrilli, Michelangelo, Widmalm, Göran, Tutino, MARIA LUISA, Corsaro, MARIA MICHELA |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Recrystallization (geology) Stereochemistry Extracellular polysaccharide 010402 general chemistry Polysaccharide 01 natural sciences Microbiology Article 03 medical and health sciences chemistry.chemical_compound QD Psychrophile Molecular Biology chemistry.chemical_classification Bacillosamine biology Strain (chemistry) Extracellular polysaccharides Chemistry Cold adaptation Kemi General Medicine Nuclear magnetic resonance spectroscopy Anti-freeze activity biology.organism_classification NMR 0104 chemical sciences 030104 developmental biology Aminosugar Chemical Sciences Bacteria |
| ISSN: | 0003-6072 |
| Popis: | Colwellia psychrerythraea strain 34H, a Gram-negative bacterium isolated from Arctic marine sediments, is considered a model to study the adaptation to cold environments. Recently, we demonstrated that C. psychrerythraea 34H produces two different extracellular polysaccharides, a capsular polysaccharide and a medium released polysaccharide, which confer cryoprotection to the bacterium. In this study, we report the structure of an additional capsular polysaccharide produced by Colwellia grown at a different temperature. The structure was determined using chemical methods, and one- and two-dimensional NMR spectroscopy. The results showed a trisaccharide repeating unit made up of only amino-sugar residues: N-acetyl-galactosamine, 2,4-diacetamido-2,4,6-trideoxy-glucose (bacillosamine), and 2-acetamido-2-deoxyglucuronic acid with the following structure: -> 4)-beta-d-GlcpNAcA-(1 -> 3)-beta-d-QuipNAc4NAc-(1 -> 3)-beta-d-GalpNAc-(1 ->. The 3D model, generated in accordance with H-1,H-1-NOE NMR correlations and consisting of ten repeating units, shows a helical structure. In contrast with the other extracellular polysaccharides produced from Colwellia at 4 A degrees C, this molecule displays only a low ice recrystallization inhibition activity. Open Access 2019-09-01 |
| Databáze: | OpenAIRE |
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