Genome Damage in Rats after Transplacental Exposure to Jatropha dioica Root Extract
| Autor: | Celia Guerrero-Velázquez, Rosalinda Gutiérrez-Hernández, Blanca Patricia Lazalde-Ramos, Susana Vanessa Sánchez de la Rosa, Belinda C. Gómez-Meda, Ana Lourdes Zamora-Perez, Guillermo Moisés Zúñiga-González, Gabriela Morales-Velazquez, Yveth Marlene Ortiz-García |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Jatropha dioica Article Subject biology Transplacental lcsh:Other systems of medicine Pharmacology biology.organism_classification medicine.disease_cause lcsh:RZ201-999 03 medical and health sciences 0302 clinical medicine Complementary and alternative medicine Oral administration In vivo 030220 oncology & carcinogenesis Micronucleus test Transplacental Exposure medicine Gestation Genotoxicity Research Article 030304 developmental biology |
| Zdroj: | Evidence-Based Complementary and Alternative Medicine, Vol 2019 (2019) Evidence-based Complementary and Alternative Medicine : eCAM |
| ISSN: | 1741-4288 |
| Popis: | Jatropha dioica is traditionally used owing to its antiviral, antifungal, and antimicrobial properties. But, toxicological information regarding J. dioica root total extract is currently limited. The aim of this work was to evaluate in a rat model, the transplacental genotoxicity effect of J. dioica aqueous root total extract. Three different J. dioica aqueous root total extract doses (60, 100, and 300 mg/kg) were administered orally to Wistar rats during 5 days through the pregnancy term (16–21 days). Pregnant rats were sampled every 24 h during the last 6 days of gestation, and pubs were sampled at birth. Genome damage in dams and their newborn pups transplacentally exposed to J. dioica was evaluated by in vivo micronuclei assay. We evaluated the frequency of micronucleated erythrocytes (MNE), micronucleated polychromatic erythrocytes (MNPCE), and polychromatic erythrocytes (PCE) in peripheral blood samples from pups and MNPCE and PCE in pregnant rats. No genotoxic effect was observed after oral administration of the three different doses of aqueous root total extract of J. dioica in pregnant or in their newborn pubs, after transplacental exposure. A significant decrease in PCE frequency was noted in samples from pubs of rats treated with the highest dose of J. dioica extract. The aqueous total root extract of J. dioica at the highest dose tested in our research do have cytotoxic effect in pups transplacentally exposed to this plant extract. Moreover, neither a genotoxic nor a cytotoxic effect was observed in pregnant rats. In the present work, there was no evidence of genome damage in the rat model after transplacental exposure to J. dioica aqueous root total extract. |
| Databáze: | OpenAIRE |
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