Development of two-photon polymerised scaffolds for optical interrogation and neurite guidance of human iPSC-derived cortical neuronal networks
| Autor: | Pablo Loza-Alvarez, Ayman El-Tamer, Anastasia Koroleva, Omar E. Olarte, Estefanía Estévez-Priego, S.G. Sokolovsky, H. R. Parri, Eric J. Hill, Jordi Soriano, Edik U. Rafailov, James A Crowe, Boris N. Chichkov, Adriaan-Alexander Ludl, Jorge Madrid-Wolff, David A. Nagel |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Complex systems
Neurite Computer science Induced Pluripotent Stem Cells Biomedical Engineering Bioengineering Biocompatible Materials 02 engineering and technology Stem cells Biochemistry 03 medical and health sciences Two-photon excitation microscopy Xarxes neuronals (Neurobiologia) Humans Progenitor cell Neural networks (Neurobiology) Induced pluripotent stem cell 030304 developmental biology Neurons 0303 health sciences Artificial neural network Tissue Scaffolds Biomaterial Cell Differentiation General Chemistry Complex network 021001 nanoscience & nanotechnology Biocompatible material Axon Guidance Sistemes complexos 0210 nano-technology Cèl·lules mare Neuroscience |
| Zdroj: | Lab on a Chip Dipòsit Digital de la UB Universidad de Barcelona |
| ISSN: | 1473-0189 |
| Popis: | Recent progress in the field of human induced pluripotent stem cells (iPSCs) has led to the efficient production of human neuronal cell models for in vitro study. This has the potential to enable the understanding of live human cellular and network function which is otherwise not possible. However, a major challenge is the generation of reproducible neural networks together with the ability to interrogate and record at the single cell level. A promising aid is the use of biomaterial scaffolds that would enable the development and guidance of neuronal networks in physiologically relevant architectures and dimensionality. The optimal scaffold material would need to be precisely fabricated with submicron resolution, be optically transparent, and biocompatible. Two-photon polymerisation (2PP) enables precise microfabrication of three-dimensional structures. In this study, we report the identification of two biomaterials that support the growth and differentiation of human iPSC-derived neural progenitors into functional neuronal networks. Furthermore, these materials can be patterned to induce alignment of neuronal processes and enable the optical interrogation of individual cells. 2PP scaffolds with tailored topographies therefore provide an effective method of producing defined in vitro human neural networks for application in influencing neurite guidance and complex network activity. publishedVersion |
| Databáze: | OpenAIRE |
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