Preparing semisynthetic and fully synthetic histones h3 and h4 to modify the nucleosome core

Autor:	Cecil J. Howard, John C. Shimko, Jennifer J. Ottesen, Michael G. Poirier
Rok vydání:	2013
Předmět:	Binding Sites biology Lysine Acetylation Native chemical ligation Article Nucleosomes Histones Histone H3 Histone Biochemistry Histone methylation biology.protein Nucleosome Histone code Chemical ligation Histone octamer Molecular Biology Protein Processing Post-Translational Protein Binding
Zdroj:	Methods in Molecular Biology ISBN: 9781627033046
ISSN:	1940-6029
Popis:	The purpose of this chapter is to provide practical chemical ligation procedures to prepare histone proteins suitable for the reconstitution of nucleosomes with specific posttranslational modifications in the nucleosome core. Detailed methods are described for the efficient preparation of semi synthetic histones H3 and H4 with modifications near the C-terminus of the proteins by expressed protein ligation and desulfurization. Additionally, we present optimized protocols for solid phase peptide synthesis combined with sequential native chemical ligation to generate fully synthetic modified histone H3, here in the context of H3 lysine 56 acetylation (H3K56ac).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb03ee7f6c53e3154eeafd62935ffbdb https://pubmed.ncbi.nlm.nih.gov/23381862 Zobrazit plný text záznamu