Anticancer potential of ZnO nanoparticle-ferulic acid conjugate on Huh-7 and HepG2 cells and diethyl nitrosamine induced hepatocellular cancer on Wistar albino rat
| Autor: | Bharathidasan Rajamani, Premkumar Kumpati, Suresh Kumar Muthuvel, Suyavaran Arumugam, Thirunavukkarasu Chinnasamy, Subastri Ariraman, Nithyananthan Subramaniyan, Preedia Babu Ezhuthupurakkal |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Alkylating Agents Programmed cell death Carcinoma Hepatocellular Coumaric Acids Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Antineoplastic Agents Apoptosis Bioengineering 02 engineering and technology Ferulic acid 03 medical and health sciences chemistry.chemical_compound Liver Neoplasms Experimental Western blot In vivo Tumor Cells Cultured medicine Animals Humans Diethylnitrosamine General Materials Science Viability assay Rats Wistar Cell Proliferation medicine.diagnostic_test Chemistry Hep G2 Cells 021001 nanoscience & nanotechnology In vitro Rats Comet assay 030104 developmental biology Biochemistry Cancer research Nanoparticles Molecular Medicine Zinc Oxide 0210 nano-technology |
| Zdroj: | Nanomedicine: Nanotechnology, Biology and Medicine. 14:415-428 |
| ISSN: | 1549-9634 |
| DOI: | 10.1016/j.nano.2017.11.003 |
| Popis: | Drawbacks and limitations of recently available therapies to hepatocellular cancer (HCC) devoted the scientist to focus on emerging new strategies. ZnO nanoparticles (ZnONPs) based chemotherapeutics has been emanating as a promising approach to maximize therapeutic synergy facilitating the discovery of novel multitargeted combinations. In the present study we conjugated ZnONPs with ferulic acid (ZnONPs-FAC) characterized by computational, spectroscopic and microscopic techniques. In vitro anticancer potential has been evaluated by assessing cell viability, morphology, ROS generation, mitochondrial membrane permeability, comet assay, immunofluorescent staining of 8-OHdG, Ki67 and γ-H2AX, cell cycle analysis and western blot analysis and in vivo anticancer potential against DEN induced HCC was analyzed by histopathological and immunohistochemical methods. The results revealed that ZnONPs-FAC induces cell death through apoptosis and can suppress the DEN-induced HCC. Our study documents therapeutic potential of nanoparticle conjugated with phytochemicals, suggesting a new platform for combinatorial chemotherapy. |
| Databáze: | OpenAIRE |
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