Epidermal neural crest stem cell transplantation as a promising therapeutic strategy for ischemic stroke

Autor: Negar Azarpira, Mohammad Saied Salehi, Seyed Mostafa Shid Moosavi, Sadegh Izadi, Anahid Safari, Somayeh Nazari, Shahrokh Zare, Leila Dargahi, Maryam Poursadeghfard, Afshin Borhani-Haghighi, Benjamin Jurek, Somaye Keshavarz, Amin Tamadon, Mojdeh Heidari, Zahra Khodabandeh, Sareh Pandamooz, Mohammad Reza Namavar, Mehdi Dianatpour, Sadegh Fattahi
Rok vydání: 2020
Předmět:
Male
0301 basic medicine
Pathology
medicine.medical_specialty
Doublecortin Protein
Ischemia
cerebral ischemia
Brain Ischemia
Rats
Sprague-Dawley
Cell therapy
03 medical and health sciences
0302 clinical medicine
Neural Stem Cells
Physiology (medical)
medicine
Glial cell line-derived neurotrophic factor
Animals
Pharmacology (medical)
neurological deficits
Stroke
Ischemic Stroke
Pharmacology
biology
business.industry
bone marrow mesenchymal stem cells
epidermal neural crest stem cells
Original Articles
Nestin
medicine.disease
Rats
Doublecortin
Transplantation
Psychiatry and Mental health
Infarct volume
030104 developmental biology
Epidermal Cells
nervous system
Neural Crest
biology.protein
Original Article
cell therapy
Stem cell
business
030217 neurology & neurosurgery
Stem Cell Transplantation
Zdroj: CNS Neuroscience & Therapeutics
ISSN: 1755-5949
1755-5930
DOI: 10.1111/cns.13370
Popis: Introduction Cell‐based therapy is considered as promising strategy to cure stroke. However, employing appropriate type of stem cell to fulfill many therapeutic needs of cerebral ischemia is still challenging. In this regard, the current study was designed to elucidate therapeutic potential of epidermal neural crest stem cells (EPI‐NCSCs) compared to bone marrow mesenchymal stem cells (BM‐MSCs) in rat model of ischemic stroke. Methods Ischemic stroke was induced by middle cerebral artery occlusion (MCAO) for 45 minutes. Immediately after reperfusion, EPI‐NCSCs or BM‐MSCs were transplanted via intra‐arterial or intravenous route. A test for neurological function was performed before ischemia and 1, 3, and 7 days after MCAO. Also, infarct volume ratio and relative expression of 15 selected target genes were evaluated 7 days after transplantation. Results EPI‐NCSCs transplantation (both intra‐arterial and intravenous) and BM‐MSCs transplantation (only intra‐arterial) tended to result in a better functional outcome, compared to the MCAO group; however, this difference was not statistically significant. The infarct volume ratio significantly decreased in NCSC‐intra‐arterial, NCSC‐intravenous and MSC‐intra‐arterial groups compared to the control. EPI‐NCSCs interventions led to higher expression levels of Bdnf, nestin, Sox10, doublecortin, β‐III tubulin, Gfap, and interleukin‐6, whereas neurotrophin‐3 and interleukin‐10 were decreased. On the other hand, BM‐MSCs therapy resulted in upregulation of Gdnf, β‐III tubulin, and Gfap and down‐regulation of neurotrophin‐3, interleukin‐1, and interleukin‐10. Conclusion These findings highlight the therapeutic effects of EPI‐NCSCs transplantation, probably through simultaneous induction of neuronal and glial formation, as well as Bdnf over‐expression in a rat model of ischemic stroke.
Databáze: OpenAIRE
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