Rhodiola crenulata inhibits Wnt/β-catenin signaling in glioblastoma
| Autor: | Kaitlyn E. Wong, Michael V. Tirabassi, Sallie S. Schneider, Maria Carmen Mora, Richard B. Arenas, Lotfi M. Bassa |
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| Rok vydání: | 2015 |
| Předmět: |
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Western Immunocytochemistry Real-Time Polymerase Chain Reaction Plant Roots Cell Line Tumor Neurosphere Rhodiola Biomarkers Tumor Humans Clonogenic assay Wnt Signaling Pathway Cell Proliferation Glial fibrillary acidic protein biology Brain Neoplasms Plant Extracts Cell growth Wnt signaling pathway biology.organism_classification Immunohistochemistry Molecular biology Treatment Outcome Immunology biology.protein Surgery Signal transduction Glioblastoma Phytotherapy |
| Zdroj: | Journal of Surgical Research. 197:247-255 |
| ISSN: | 0022-4804 |
| DOI: | 10.1016/j.jss.2015.02.074 |
| Popis: | Background Rhodiola crenulata is a perennial plant that grows in the high altitudes of Eastern Europe and Asia. R crenulata has been used for many years in Eastern traditional medicine for a variety of medicinal purposes and it has been shown to elicit antineoplastic effects. The purpose of this study is to determine if R crenulata extract exhibits antitumor properties on glioblastoma multiforme (GBM), the most common and aggressive primary brain tumor. Materials and Methods Human U87 GBM cells were treated with 200 μg/mL of R crenulata or vehicle control. Cell proliferation was measured via MTS assay and clonogenic assay. The expressions of glial fibrillary acidic protein, a protein marker of differentiation, E-cadherin, and non–phospho active β-catenin were measured with immunocytochemistry. Neurosphere assay was performed in low attachment plates. Activity of the Wnt/β-catenin transcriptional activation was assessed via a dual-luciferase assay. Results MTS and clonogenicity assays revealed a decrease in proliferation with R crenulata therapy with an increased sensitivity to radiation. Immunocytochemistry revealed that R crenulata induced glial fibrillary acidic protein and E-cadherin expression suggestive of a more differentiated state. In agreement with the change in differentiation neurosphere formation was decreased upon treatment with R crenulata. β-Catenin dual reporter assay revealed a decrease in Wnt promoter activity after treatment with R crenulata; this was supported by a decrease in nuclear localization of β-catenin. Conclusions Treatment with R crenulata extract effectively suppresses proliferation, stimulates differentiation, and eliminates tumorsphere formation of GBM cells in vitro . The observed effects are associated with inhibition of Wnt/β-catenin signaling pathway. |
| Databáze: | OpenAIRE |
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