Diagnostic value of CD103 expression in bronchoalveolar lymphocytes in sarcoidosis
| Autor: | Natália Melo, Luís Delgado, Carmo Palmares, Marília Beltrão, Patrícia Caetano Mota, Ana Cristina Santos, António Morais |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty Sarcoidosis animal diseases CD4-CD8 Ratio chemical and pharmacologic phenomena CD8-Positive T-Lymphocytes Sensitivity and Specificity Sarcoidosis Pulmonary Antigen Antigens CD medicine Humans CD103+ Lung medicine.diagnostic_test business.industry Bronchoalveolar lavage fluid Case-control study hemic and immune systems Diagnostic marker Middle Aged respiratory system medicine.disease respiratory tract diseases Bronchoalveolar lavage medicine.anatomical_structure ROC Curve Case-Control Studies Immunology Female business Integrin alpha Chains Biomarkers CD8 |
| Zdroj: | Respiratory Medicine. 106:1014-1020 |
| ISSN: | 0954-6111 |
| DOI: | 10.1016/j.rmed.2012.03.020 |
| Popis: | SummaryBackgroundPulmonary sarcoidosis is frequently characterized by a CD4+/CD8+ ratio ≥3.5 in bronchoalveolar lavage fluid (BALF), although up to 40% of the cases present a normal or even decreased ratio, pointing out its variability and limitation as a diagnostic marker for sarcoidosis. Lung lymphocytes within the bronchial epithelium, the alveolar walls, and BALF express the integrin CD103. Our aim was to compare the expression of CD103 in BALF T-lymphocytes between sarcoidosis and other interstitial lung diseases (ILD) and to evaluate its relevance as a BALF diagnostic marker for sarcoidosis.MethodsA total of 86 patients with ILD (mean age ± standard deviation, 42.6 ± 16.6 years; 60.5% female), who underwent BALF as part of their initial diagnostic work-up, were enrolled into 2 groups: sarcoidosis (n = 41) and other ILD (n = 45). Area under the receiver operating characteristic (ROC) curve (AUC) was used to describe the performance of CD103 for sarcoidosis diagnosis.ResultsSarcoidosis patients presented a significantly reduced CD103 expression in BALF T-lymphocytes, more pronounced in the CD4+ subset. The BALF CD103+CD4+/CD4+ ratio for a cutoff point of 0.45 was associated with a better diagnostic performance for sarcoidosis (AUC: 0.86 [95% confidence interval (95% CI): 0.78–0.94]; sensitivity: 81%; specificity: 78%), even for those with a CD4+/CD8+ ratio |
| Databáze: | OpenAIRE |
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