Detection of hepatitis B virus DNA in hepatocytes, bile duct epithelium, and vascular elements by in situ hybridization
| Autor: | Linda Stowring, Hubert E. Blum, Ashley T. Haase, Girish N. Vyas, Annalena Figus, Carolyn K. Montgomery |
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| Rok vydání: | 1983 |
| Předmět: |
Hepatitis B virus
Bile Duct Epithelium Viral transformation In situ hybridization Biology medicine.disease_cause Epithelium Pathogenesis Mice Nucleic acid thermodynamics medicine Animals Humans Multidisciplinary Bile duct Nucleic Acid Hybridization virus diseases Virology Molecular biology digestive system diseases medicine.anatomical_structure Liver DNA Viral Bile Ducts Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 80:6685-6688 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.80.21.6685 |
| Popis: | A radiolabeled probe specific for hepatitis B virus (HBV) nucleotide sequences was hybridized in situ to liver tissue from three patients with chronic hepatitis B. The HBV genome was detected not only in infected hepatocytes but also in bile duct epithelial cells, endothelial cells, and smooth muscle cells. These findings extend the known host cell range for HBV, suggest new mechanisms of viral dissemination, and illustrate the usefulness of in situ hybridization in the study of pathogenesis of HBV infection. |
| Databáze: | OpenAIRE |
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