Fecal Microbiome Distinguishes Alcohol Consumption From Alcoholic Hepatitis But Does Not Discriminate Disease Severity
| Autor: | Suthat Liangpunsakul, Patrick M. Gillevet, Masoumeh Sikaroodi, Puneet Puri, Arun J. Sanyal, Vijay H. Shah, Robert E. Brown, Mark D. Muthiah, Faridoddin Mirshahi, Ekaterina Smirnova, Kayla Gelow, Sherry Boyett, Kalyani Daitya, Naga Chalasani, Mohammed S. Siddiqui |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male 0301 basic medicine medicine.medical_specialty Alcohol Drinking Firmicutes Alcoholic hepatitis Severity of Illness Index Gastroenterology Article Diagnosis Differential Feces 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Humans Microbiome Liver Diseases Alcoholic Hepatitis Hepatology biology Hepatitis Alcoholic business.industry Microbiota Lachnospiraceae Gastrointestinal Microbiome Area under the curve Middle Aged biology.organism_classification medicine.disease 030104 developmental biology Female 030211 gastroenterology & hepatology business |
| Zdroj: | Hepatology |
| ISSN: | 1527-3350 0270-9139 |
| DOI: | 10.1002/hep.31178 |
| Popis: | BACKGROUND AND AIMS The role of the intestinal microbiome in alcoholic hepatitis is not established. The aims of this study were to (1) characterize the fecal microbial ecology associated with alcoholic hepatitis, (2) relate microbiome changes to disease severity, and (3) infer the functional relevance of shifts in microbial ecology. APPROACH AND RESULTS The fecal microbiome in patients with moderate alcoholic hepatitis (MAH) or severe alcoholic hepatitis (SAH) was compared with healthy controls (HCs) and heavy drinking controls (HDCs). Microbial taxa were identified by 16S pyrosequencing. Functional metagenomics was performed using PICRUSt. Fecal short chain fatty acids (SCFAs) were measured using a liquid chromatography-mass spectrometry platform. A total of 78 participants (HC, n = 24; HDC, n = 20; MAH, n = 10; SAH, n = 24) were studied. HDC had a distinct signature compared with HC with depletion of Bacteroidetes (46% vs. 26%; P = 0.01). Alcoholic hepatitis was associated with a distinct microbiome signature compared with HDC (area under the curve = 0.826); differential abundance of Ruminococcaceae, Veillonellaceae, Lachnospiraceae, Porphyromonadaceae, and Rikenellaceae families were the key contributors to these differences. The beta diversity was significantly different among the groups (permutational multivariate analysis of variance [PERMANOVA] P |
| Databáze: | OpenAIRE |
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