Frequency-Modulated Continuous Flow Analysis Electrospray Ionization Mass Spectrometry (FM-CFA-ESI-MS) for Sample Multiplexing
| Autor: | Adrian M. Schrell, James L. Edwards, John B Coulton, Robert T. Filla, Michael G. Roper |
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| Rok vydání: | 2018 |
| Předmět: |
Chemistry
Calibration curve Dynamic range Electrospray ionization 010401 analytical chemistry Analytical chemistry Ion current 02 engineering and technology 021001 nanoscience & nanotechnology Mass spectrometry 01 natural sciences 0104 chemical sciences Analytical Chemistry Ion Frequency domain Deconvolution 0210 nano-technology |
| Zdroj: | Analytical chemistry. 90(4) |
| ISSN: | 1520-6882 |
| Popis: | A method for multiplexed sample analysis by mass spectrometry without the need for chemical tagging is presented. In this new method, each sample is pulsed at unique frequencies, mixed, and delivered to the mass spectrometer while maintaining a constant total flow rate. Reconstructed ion currents are then a time-dependent signal consisting of the sum of the ion currents from the various samples. Spectral deconvolution of each reconstructed ion current reveals the identity of each sample, encoded by its unique frequency, and its concentration encoded by the peak height in the frequency domain. This technique is different from other approaches that have been described, which have used modulation techniques to increase the signal-to-noise ratio of a single sample. As proof of concept of this new method, two samples containing up to 9 analytes were multiplexed. The linear dynamic range of the calibration curve was increased with extended acquisition times of the experiment and longer oscillation periods of the samples. Because of the combination of the samples, salt had little effect on the ability of this method to achieve relative quantitation. Continued development of this method is expected to allow for increased numbers of samples that can be multiplexed. |
| Databáze: | OpenAIRE |
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