Wnt / β-Catenin-Signalweg in Fälle von hepatozellulären Karzinomen aus Kolumbien
| Autor: | Rocio Lopez, Pierre Hainaut, Iris Suarez M, Germán Osorio, Pascal Pineau, Diego Uribe, Sergio Hoyos, Juan Camilo Pérez, Maria-C Navas, Carlos Mario Jaramillo |
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| Přispěvatelé: | Grupo de Gastrohepatologia [Medellin, Colombia], Universidad de Antioquia = University of Antioquia [Medellín, Colombia], Departamento de Patologia [Medellin, Colombia], Facultad de Psicología, Valencia, Hospital Pablo Tobón Uribe [Medellín, Colombie], Fundacion Santa Fe de Bogota [Colombia], International Prevention Research Institute (IPRI), Organisation Nucléaire et Oncogenèse / Nuclear Organization and Oncogenesis, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), This study was funded by Departamento Nacional de Ciencia, Innovación y Tecnologia Colciencias (Grant 111540820471) and Proyecto de Sostenibilidad, Vicerrectoría de Investigación, Universidad de Antioquia., We thank Dr. Anne Lise Haenni from the Institut Jacques Monod for critical reading of the manuscript and Dr. Andres Arias from Universidad de Antioquia for discussion of the results. We also thank Ghyslaine Martel from International Agency for Research on Cancer (IARC) and Beatriz Vieco from Departamento de Patología, Universidad de Antioquia, for technical assistance., Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Rok vydání: | 2015 |
| Předmět: |
Pathology
MESH: Wnt Signaling Pathway / genetics Specialties of internal medicine MESH: Neoplasm Grading MESH: Colombia medicine.disease_cause Exon MESH: Aged 80 and over MESH: Liver Neoplasms / genetics CTNNB1 HCC MESH: Cohort Studies HBV infection MESH: Aged Mutation MESH: Middle Aged Subcellular localization MESH: Hepatitis B Chronic / complications Wnt signaling pathway MESH: Carcinoma Hepatocellular / pathology General Medicine MESH: beta Catenin / metabolism Hepatitis B 3. Good health RC581-951 Hepatocellular carcinoma Immunohistochemistry MESH: Liver Neoplasms / etiology medicine.medical_specialty MESH: Carcinoma Hepatocellular / etiology [SDV.CAN]Life Sciences [q-bio]/Cancer Biology [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] medicine Carcinoma neoplasms Gene MESH: Age Factors MESH: beta Catenin / genetics MESH: Liver Neoplasms / pathology MESH: Humans Hepatology MESH: Carcinoma Hepatocellular / genetics MESH: Retrospective Studies MESH: Immunohistochemistry [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology β-catenin medicine.disease MESH: Male digestive system diseases MESH: Mutation Cancer research MESH: Exons MESH: Female |
| Zdroj: | Annals of hepatology : official journal of the Mexican Association of Hepatology Annals of hepatology : official journal of the Mexican Association of Hepatology, Elsevier, 2015, 14 (1), pp.64-74. ⟨10.1016/S1665-2681(19)30802-6⟩ Annals of Hepatology, Vol 14, Iss 1, Pp 64-74 (2015) Annals of hepatology : official journal of the Mexican Association of Hepatology, 2015, 14 (1), pp.64-74. ⟨10.1016/S1665-2681(19)30802-6⟩ |
| ISSN: | 1665-2681 |
| DOI: | 10.1016/s1665-2681(19)30802-6 |
| Popis: | International audience; Background and aim. Hepatocellular carcinoma (HCC) is the most common primary liver cancer diagnosed worldwide. Deregulation of Wnt/β-catenin pathway has been associated with the development of HCC in a substantial number of cases in Europe and far less in Asia. Nothing is known about this pathway in HCC cases from South America. This study aimed to investigate the frequency of mutations in β-catenin gene (CTNNB1) and the subcellular localization of β-catenin in HCC cases from Colombia. Material and methods. We determine by direct sequencing the frequency of mutations in exon 3 of CTNNB1 gene and by immu-nohistochemistry the subcellular localization of β-catenin in 54 samples of HCC obtained from three pathology units in Bogota and Medellin cities. Results. Only three HCC cases (5.6%) were found mutated at residues (G34E, S45P, P44S, T41I) important for phosphorylation and ubiquitination of β-catenin protein. Strikingly, nuclear or cytoplasmic accumulation of β-catenin, hallmark of Wnt pathway activation, was found in 42.6% HCC cases (23/54). Interestingly, β-catenin accumulation was significantly more frequent in young patients and hepatitis B virus-related HCC. Conclusions. Although, CTNNB1 exon 3 mutations are not frequent in HCC from Colombian patients, our findings indicate that Wnt/β-catenin signaling is activated in 42.6% of HCC samples. Furthermore, Wnt signaling was demonstrated in HCC cases associated of HBV infection, one of the most important HCC risk factors in Colombia. |
| Databáze: | OpenAIRE |
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