Quantitative microautoradiography of arteries: comparison of radioactivity to silver

Autor:	S. Y. Oh, D. L. Fry, C. R. Swyt, Robert W. Mahley, S. J. Lewis, F. Plowman
Rok vydání:	1980
Předmět:	Silver Histocytochemistry Physiology Chemistry Electron Spin Resonance Spectroscopy Analytical chemistry Arteries Microanalysis law.invention Iodine Radioisotopes Dogs law Physiology (medical) Microtome Animals Autoradiography Cardiology and Cardiovascular Medicine Serum Albumin
Zdroj:	American Journal of Physiology-Heart and Circulatory Physiology. 239:H289-H295
ISSN:	1522-1539 0363-6135
DOI:	10.1152/ajpheart.1980.239.2.h289
Popis:	The local concentration of silver in developed aortic transmural microautoradiographs was compared to the corresponding 125I-labeled albumin radioactivity concentration [ci(x)] in the subjacent tissue. Silver [s(x)] was measured by electron probe X-ray microanalysis (EPA) and the corresponding ci(x) by direct gamma-ray counting. The results show 1) that the relationship between volume-averaged values of radioactivity (ci) and EPA signal (s) is adequately described by ci = Ks, where K is a proportionality constant, and 2) that ci(x) measured by EPA [i.e., Ks(x)] agrees closely with ci(s) measured direcly from en face microtomy slices of corresponding unfixed tissue specimens.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::baf9e23c2b5690c549f589c37a21730c https://doi.org/10.1152/ajpheart.1980.239.2.h289 Zobrazit plný text záznamu