Constructing Biopolymer-Inorganic Nanocomposite through a Biomimetic Mineralization Process for Enzyme Immobilization
| Autor: | Yanhuan Wang, Tao Jiang, Xue Mei Wen, Guozhu Li, Jun Ma, Jian Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Materials science
Immobilized enzyme biomimetic mineralization Nanotechnology engineering.material lcsh:Technology Micelle Article nanocomposite enzyme immobilization silica alginate template General Materials Science lcsh:Microscopy lcsh:QC120-168.85 chemistry.chemical_classification Nanocomposite Aqueous solution lcsh:QH201-278.5 lcsh:T Polymer Mineralization (soil science) chemistry lcsh:TA1-2040 engineering lcsh:Descriptive and experimental mechanics Cage effect lcsh:Electrical engineering. Electronics. Nuclear engineering Biopolymer lcsh:Engineering (General). Civil engineering (General) lcsh:TK1-9971 |
| Zdroj: | Materials Volume 8 Issue 9 Pages 6004-6017 Materials; Volume 8; Issue 9; Pages: 6004-6017 Materials, Vol 8, Iss 9, Pp 6004-6017 (2015) |
| ISSN: | 1996-1944 |
| Popis: | Inspired by biosilicification, biomimetic polymer-silica nanocomposite has aroused a lot of interest from the viewpoints of both scientific research and technological applications. In this study, a novel dual functional polymer, NH2-Alginate, is synthesized through an oxidation-amination-reduction process. The “catalysis function” ensures the as-prepared NH2-Alginate inducing biomimetic mineralization of silica from low concentration precursor (Na2SiO3), and the “template function” cause microscopic phase separation in aqueous solution. The diameter of resultant NH2-Alginate micelles in aqueous solution distributed from 100 nm to 1.5 μm, and is influenced by the synthetic process of NH2-Alginate. The size and morphology of obtained NH2-Alginate/silica nanocomposite are correlated with the micelles. NH2-Alginate/silica nanocomposite was subsequently utilized to immobilize β-Glucuronidase (GUS). The harsh condition tolerance and long-term storage stability of the immobilized GUS are notably improved due to the buffering effect of NH2-Alginate and cage effect of silica matrix. |
| Databáze: | OpenAIRE |
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