Dysregulation of hepatic microRNA expression in C57BL/6 mice affected by excretory-secretory products of Fasciola gigantica

Autor: Ke-Jing Lu, Hany M. Elsheikha, Zhao-An Sheng, Wei Shi, Weiyi Huang, Yaoyao Zhang, Xue-Fang Mei, Xing-Quan Zhu, Jun-Jun He, Zi-Xuan Zeng
Přispěvatelé: Suttiprapa, Sutas
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Small RNA
Molecular biology
Physiology
RC955-962
Biochemistry
Nervous System
Lung and Intrathoracic Tumors
Pathogenesis
Mice
0302 clinical medicine
Sequencing techniques
Medical Conditions
Thymic Tumors
Arctic medicine. Tropical medicine
Medicine and Health Sciences
Endocrine Tumors
Immune Response
Prostate Cancer
Prostate Diseases
High-Throughput Nucleotide Sequencing
RNA sequencing
Cell biology
Nucleic acids
Electrophysiology
Infectious Diseases
Real-time polymerase chain reaction
Liver
Oncology
Female
Anatomy
Public aspects of medicine
RA1-1270
Research Article
Fascioliasis
Fasciola gigantica
Urology
030231 tropical medicine
Immunology
Down-Regulation
Neurophysiology
Biology
Real-Time Polymerase Chain Reaction
03 medical and health sciences
Ion binding
microRNA
Genetics
Parasitic Diseases
Animals
KEGG
Non-coding RNA
Colorectal Cancer
Natural antisense transcripts
Biology and life sciences
Carcinoma
Public Health
Environmental and Occupational Health
Immunity
Thyroid Carcinoma
Computational Biology
Cancers and Neoplasms
biology.organism_classification
Reverse transcriptase
Fasciola
Gene regulation
Mice
Inbred C57BL
Research and analysis methods
MicroRNAs
Genitourinary Tract Tumors
030104 developmental biology
Molecular biology techniques
Synapses
RNA
Gene expression
Neuroscience
Zdroj: PLoS Neglected Tropical Diseases, Vol 14, Iss 12, p e0008951 (2020)
PLoS Neglected Tropical Diseases
ISSN: 1935-2735
1935-2727
Popis: The excretory-secretory products released by the liver fluke Fasciola gigantica (FgESPs) play important roles in regulating the host immune response during the infection. Identification of hepatic miRNAs altered by FgESPs may improve our understanding of the pathogenesis of F. gigantica infection. In this study, we investigated the alterations in the hepatic microRNAs (miRNAs) in mice treated with FgESPs using high-throughput small RNA (sRNA) sequencing and bioinformatics analysis. The expression of seven miRNAs was confirmed by quantitative stem-loop reverse transcription quantitative PCR (qRT-PCR). A total of 1,313 miRNAs were identified in the liver of mice, and the differentially expressed (DE) miRNAs varied across the time lapsed post exposure to FgESPs. We identified 67, 154 and 53 dysregulated miRNAs at 1, 4 and 12 weeks post-exposure, respectively. 5 miRNAs (miR-126a-3p, miR-150-5p, miR-155-5p, miR-181a-5p and miR-362-3p) were commonly dysregulated at the three time points. We also found that most of the DE miRNAs were induced by FgESPs in the mouse liver after 4 weeks of exposure. These were subjected to Gene Ontology (GO) enrichment analysis, which showed that the predicted targets of the hepatic DE miRNAs of mice 4 weeks of FgESPs injection were enriched in GO terms, including cell membrane, ion binding, cellular communication, organelle and DNA damage. KEGG analysis indicated that the predicted targets of the most downregulated miRNAs were involved in 15 neural activity-related pathways, 6 digestion-related pathways, 20 immune response-related pathways and 17 cancer-related pathways. These data provide new insights into how FgESPs can dysregulate hepatic miRNAs, which play important roles in modulating several aspects of F. gigantica pathogenesis.
Author summary Fasciolosis is a worldwide neglected parasitic disease which is caused by the infections of liver flukes, such as Fasciola hepatica and Fasciola gigantica. The excretory-secretory products released by F. gigantica (FgESPs) play important roles in regulating host biological processes. However, the regulation mechanism remains unclear. This study used high-throughput RNA sequencing to describe the comprehensive small RNA profile altered by FgESPs stimulation. We manifested that weeks of FgESPs exposure lead to a possible hypo-reactiveness and the small RNA profile was significantly altered, especially at 4 weeks post exposure. GO and KEGG pathway analyses of DEmiRNAs targets showed that FgESPs participate in regulation of neural activities, digestive function, and immune responses of host, alter several host cellular components or functions such as cell membrane, cellular communication, organelle and checking of DNA damage. The findings of this study reveal the small RNA profile changes following FgESPs stimulation, providing novel data for elucidating the interaction between Fasciola gigantica and infected host.
Databáze: OpenAIRE
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