Isolation and preservation of peripheral blood mononuclear cells for analysis of islet antigen-reactive T cell responses: position statement of the T-Cell Workshop Committee of the Immunology of Diabetes Society
| Autor: | R, Mallone, S I, Mannering, B M, Brooks-Worrell, I, Durinovic-Belló, C M, Cilio, F S, Wong, N C, Schloot, Susan, Wong |
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| Rok vydání: | 2010 |
| Předmět: |
T-Lymphocytes
T cell Immunology Cell Separation Infections Peripheral blood mononuclear cell Monocytes Autoimmune Diseases Blood cell Mice Immune system Antigen Insulin-Secreting Cells Neoplasms medicine Animals Humans Immunology and Allergy Review Articles Cryopreservation Autoimmune disease Clinical Trials as Topic business.industry ELISPOT T lymphocyte medicine.disease Diabetes Mellitus Type 1 medicine.anatomical_structure Blood Preservation Antigens Surface business |
| Zdroj: | Clinical and Experimental Immunology. 163:33-49 |
| ISSN: | 1365-2249 0009-9104 |
| Popis: | Summary Autoimmune T cell responses directed against insulin-producing β cells are central to the pathogenesis of type 1 diabetes (T1D). Detection of such responses is therefore critical to provide novel biomarkers for T1D ‘immune staging’ and to understand the mechanisms underlying the disease. While different T cell assays are being developed for these purposes, it is important to optimize and standardize methods for processing human blood samples for these assays. To this end, we review data relevant to critical parameters in peripheral blood mononuclear cell (PBMC) isolation, (cryo)preservation, distribution and usage for detecting antigen-specific T cell responses. Based on these data, we propose recommendations on processing blood samples for T cell assays and identify gaps in knowledge that need to be addressed. These recommendations may be relevant not only for the analysis of T cell responses in autoimmune disease, but also in cancer and infectious disease, particularly in the context of clinical trials. |
| Databáze: | OpenAIRE |
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