Adoptive transfer of autologous Epstein-Barr virus-specific cytotoxic T cells for nasopharyngeal carcinoma
| Autor: | Daniel Chua, Stanley W.K. Im, Jonathan S.T. Sham, Mun-Hon Ng, Bo-Jian Zheng, Denis J. Moss, Winsie Luk, See Yan Lau, Kwok-Yung Yuen, Dora L.W. Kwong, Jie Huang |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male Cancer Research Adoptive cell transfer Cellular immunity Herpesvirus 4 Human medicine.medical_treatment Lymphoproliferative disorders medicine.disease_cause Immune system medicine Tumor Cells Cultured Humans business.industry Nasopharyngeal Neoplasms Immunotherapy Middle Aged medicine.disease Hematopoietic Stem Cells Epstein–Barr virus Adoptive Transfer Oncology Nasopharyngeal carcinoma Immunology Female Viral disease business T-Lymphocytes Cytotoxic |
| Zdroj: | International journal of cancer. 94(1) |
| ISSN: | 0020-7136 |
| Popis: | Tumor cells from NPC patients are regularly and latently infected with EBV. To examine whether the virus serves as target for immune intervention of the cancer, we determined levels of EBV-specific CTLp in peripheral blood from NPC patients, long-term survivors of the cancer and healthy subjects. CTLp levels of test subjects varied between 3-3,000/10(6) PBMCs. The plasma EBV burden increased when the CTLp level fell below 150, whereas the EBV burden of PBMCs was not correlated with CTLp level. Compared with healthy carriers, CTLp levels of patients were lower and varied over a wider range, between 3-1,500/10(6) PBMCs. The quantitative immune deficit was probably attributed to the tumor because, first, CTLp in survivors was restored to levels similar to those in healthy carriers after the tumor had been successfully treated. Second, the CTLp level changed as disease progressed, being lower in local disease, increased in locoregional disease and decreased again when the tumor metastasized. Based on these findings, 4 patients with advanced disease were infused with 5 x 10(7)-3 x 10(8) autologous EBV CTLs. The treatment was safe and unaccompanied by inflammatory or other complications, but whether it improved tumor control could not be discerned from the large tumor bulk. Nevertheless, the treatment regularly increased CTLp levels of patients, maintained it at higher levels for protracted periods and, in 3 patients, restored host surveillance of EBV replication, reducing the plasma EBV burden. Taken together, these results provided a rationale to further explore EBV as a target of immune intervention of NPC. (C) 2001 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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