The 5′ Repeat Elements of the Mouse Xist Gene Inhibit the Transcription of X-Linked Genes
| Autor: | Bonny C, Djemaï A, Schorderet Df, Nathalie Allaman-Pillet |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Hypoxanthine Phosphoribosyltransferase
RNA Untranslated X Chromosome Transcription Genetic Genetic Linkage Blotting Western Glucosephosphate Dehydrogenase Biology Article X-inactivation Mice Tandem repeat Transcription (biology) Genetics Animals Nuclear protein Molecular Biology Gene X chromosome DNA Primers Base Sequence Reverse Transcriptase Polymerase Chain Reaction RNA Blotting Northern Phosphoglycerate Kinase RNA Long Noncoding XIST Transcription Factors |
| Zdroj: | Gene Expression. 9:93-101 |
| ISSN: | 1555-3884 1052-2166 |
| DOI: | 10.3727/000000001783992632 |
| Popis: | X chromosome inactivation in mammals requires the Xist gene, which is exclusively expressed from the inactive X chromosome (Xi). The large heterogeneous Xist nuclear RNA colocalizes with Xi, most likely through nuclear protein interactions. The 5′ region of the Xist RNA contains a series of well-conserved tandem repeats known to bind heteronuclear proteins in vitro and to enhance human XIST transcription. We show in an in vitro system that the conserved repeat element located in the 5′ region of the mouse Xist gene (Xcr) represses three X-linked genes but has no effect on the autosomal genes Aprt, Ins, and the viral SV40 gene. The repression effect is not mediated by the conserved core sequence (Ccs) of Xcr, but requires the presence of the complete Xcr. This Xcr effect on X-linked genes suggests that Xcr transcript recognizes the genes to be silenced and is involved in the spreading of X inactivation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|