FXR1P But Not FMRP Regulates the Levels of Mammalian Brain-Specific microRNA-9 and microRNA-124
| Autor: | Zhe Fang, Ruiting Zong, Fen-Biao Gao, David L. Nelson, Zhaodong Li, Xia-Lian Xu, Helal Uddin Biswas |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Ribonuclease III Article DEAD-box RNA Helicases Fragile X Mental Retardation Protein Mice Downregulation and upregulation In vivo microRNA Animals Humans Cells Cultured Mice Knockout biology General Neuroscience HEK 293 cells Brain RNA-Binding Proteins FMR1 Molecular biology In vitro Cell biology MicroRNAs HEK293 Cells Fragile X Syndrome biology.protein Female Function (biology) Dicer |
| Zdroj: | The Journal of Neuroscience. 31:13705-13709 |
| ISSN: | 1529-2401 0270-6474 |
| DOI: | 10.1523/jneurosci.2827-11.2011 |
| Popis: | Mammalian brain-specific miR-9 and miR-124 have been implicated in several aspects of neuronal development and function. However, it is not known how their expression levels are regulatedin vivo. We found that the levels of miR-9 and miR-124 are regulated by FXR1P but not by the loss of FXR2P or FMRPin vivo, a mouse model of fragile X syndrome. Surprisingly, the levels of miR-9 and miR-124 are elevated infmr1/fxr2double-knock-out mice, in part reflecting posttranscriptional upregulation of FXR1P. Indeed, FXR1P is required for efficient processing of pre-miR-9 and pre-miR-124in vitroand forms a complex with Dicer and pre-miRNAs. These findings reveal differential roles of FMRP family proteins in controlling the expression levels of brain-specific miRNAs. |
| Databáze: | OpenAIRE |
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