Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia
| Autor: | V. N. Azevedo, M.I.M. Souza, Sandra Souza Lima, Núbia Caroline Costa de Almeida, M.M. Zaninotto, Luiz Fernando Almeida Machado, Samara Tatielle Monteiro Gomes, M.A.A. Fossa, Rosimar Neris Martins-Feitosa, Maria Alice Freitas Queiroz, Ricardo Ishak, Renata B. Hermes, M. O. G. Ishak, M.A. Maneschy, S.R.C.F. Costa, Antonio C. R. Vallinoto |
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| Jazyk: | angličtina |
| Předmět: |
Male
0301 basic medicine Genotype Heart disease Physiology Immunology Heart Valve Diseases Biophysics Ocean Engineering Single-nucleotide polymorphism Biology Polymerase Chain Reaction Polymorphism Single Nucleotide Biochemistry 03 medical and health sciences Gene Frequency medicine Humans Genetic Predisposition to Disease General Pharmacology Toxicology and Pharmaceutics Allele Chlamydia Allele frequency lcsh:QH301-705.5 Mannan-binding lectin Mannose-binding lectin lcsh:R5-920 General Neuroscience Biomedical Sciences Cell Biology General Medicine Chlamydia Infections Middle Aged medicine.disease Molecular biology Cross-Sectional Studies 030104 developmental biology Real-time polymerase chain reaction lcsh:Biology (General) Case-Control Studies Female Polymorphisms lcsh:Medicine (General) |
| Zdroj: | Brazilian Journal of Medical and Biological Research, Vol 49, Iss 12 Brazilian Journal of Medical and Biological Research, Volume: 49, Issue: 12, Article number: e5519, Published: 12 DEC 2016 Brazilian Journal of Medical and Biological Research v.49 n.12 2016 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
| Popis: | The presence of the single nucleotide polymorphisms in exon 1 of the mannose-binding lectin 2 (MBL2) gene was evaluated in a sample of 159 patients undergoing coronary artery bypass surgery (71 patients undergoing valve replacement surgery and 300 control subjects) to investigate a possible association between polymorphisms and heart disease with Chlamydia infection. The identification of the alleles B and D was performed using real time polymerase chain reaction (PCR) and of the allele C was accomplished through PCR assays followed by digestion with the restriction enzyme. The comparative analysis of allelic and genotypic frequencies between the three groups did not reveal any significant difference, even when related to previous Chlamydia infection. Variations in the MBL plasma levels were influenced by the presence of polymorphisms, being significantly higher in the group of cardiac patients, but without representing a risk for the disease. The results showed that despite MBL2 gene polymorphisms being associated with the protein plasma levels, the polymorphisms were not enough to predict the development of heart disease, regardless of infection with both species of Chlamydia. |
| Databáze: | OpenAIRE |
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