Microbial communities in full-scale woodchip bioreactors treating aquaculture effluents
| Autor: | Suvi Suurnäkki, Marja Tiirola, Sanni L. Aalto, Per Bovbjerg Pedersen, Mathis von Ahnen |
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| Rok vydání: | 2021 |
| Předmět: |
denitrifikaatio
sulfidit Environmental Engineering Denitrification Aquaculture 010501 environmental sciences Management Monitoring Policy and Law complex mixtures 01 natural sciences 03 medical and health sciences Bioreactors RNA Ribosomal 16S Bioreactor 14. Life underwater Autotroph Sulfate-reducing bacteria vesiviljely (kalatalous) Waste Management and Disposal Effluent hake jäteveden käsittely 030304 developmental biology 0105 earth and related environmental sciences 0303 health sciences Nitrates biology Microbiota Fungi technology industry and agriculture Recirculating aquaculture system General Medicine equipment and supplies Pulp and paper industry biology.organism_classification Desulfovibrio 6. Clean water mikrobisto 13. Climate action bioreaktorit Biofilter Sulfate reduction Environmental science Microbiome sienet |
| Zdroj: | Aalto, S L, Suurnäkki, S, von Ahnen, M, Tiirola, M & Pedersen, P B 2022, ' Microbial communities in full-scale woodchip bioreactors treating aquaculture effluents ', Journal of Environmental Management, vol. 301, 113852 . https://doi.org/10.1016/j.jenvman.2021.113852 |
| ISSN: | 1095-8630 |
| Popis: | Woodchip bioreactors are being successfully applied to remove nitrate from commercial land-based recirculating aquaculture system (RAS) effluents. In order to understand and optimize the overall function of these bioreactors, knowledge on the microbial communities, especially on the microbes with potential for production or mitigation of harmful substances (e.g. hydrogen sulfide; H2S) is needed. In this study, we quantified and characterized bacterial and fungal communities, including potential H2S producers and consumers, using qPCR and high throughput sequencing of 16S rRNA gene. We took water samples from bioreactors and their inlet and outlet, and sampled biofilms growing on woodchips and on the outlet of the three full-scale woodchip bioreactors treating effluents of three individual RAS. We found that bioreactors hosted a high biomass of both bacteria and fungi. Although the composition of microbial communities of the inlet varied between the bioreactors, the conditions in the bioreactors selected for the same core microbial taxa. The H2S producing sulfate reducing bacteria (SRB) were mainly found in the nitrate-limited outlets of the bioreactors, the main groups being deltaproteobacterial Desulfobulbus and Desulfovibrio. The abundance of H2S consuming sulfate oxidizing bacteria (SOB) was 5–10 times higher than that of SRB, and SOB communities were dominated by Arcobacter and other genera from phylum Epsilonbacteraeota, which are also capable of autotrophic denitrification. Indeed, the relative abundance of potential autotrophic denitrifiers of all denitrifier sequences was even 54% in outlet water samples and 56% in the outlet biofilm samples. Altogether, our results show that the highly abundant bacterial and fungal communities in woodchip bioreactors are shaped through the conditions prevailing within the bioreactor, indicating that the bioreactors with similar design and operational settings should provide similar function even when conditions in the preceding RAS would differ. Furthermore, autotrophic denitrifiers can have a significant role in woodchip biofilters, consuming potentially produced H2S and removing nitrate, lengthening the operational age and thus further improving the overall environmental benefit of these bioreactors. |
| Databáze: | OpenAIRE |
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