Adaptive Mutations in RNA Polymerase and the Transcriptional Terminator Rho Have Similar Effects on Escherichia coli Gene Expression
| Autor: | Alejandra Rodríguez-Verdugo, Jagdish Suresh Patel, Brandon S. Gaut, Shaun M. Hug, Andrea González-González |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
relative fitness Mutant adaptive pathways Biology Evolution Molecular phenotypic convergence 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine RNA polymerase Gene expression Escherichia coli Genetics experimental evolution protein structure Molecular Biology Gene Discoveries Ecology Evolution Behavior and Systematics 030304 developmental biology Terminator Regions Genetic Evolutionary Biology 0303 health sciences transcriptional termination Escherichia coli Proteins DNA-Directed RNA Polymerases Gene Expression Regulation Bacterial rpoB Adaptation Physiological Phenotype 030104 developmental biology Terminator (genetics) chemistry Mutation Epistasis Biochemistry and Cell Biology 030217 neurology & neurosurgery |
| Zdroj: | Molecular Biology and Evolution González-González, Andrea; Hug, Shaun M; Rodríguez-Verdugo, Alejandra; Patel, Jagdish Suresh; & Gaut, Brandon S. (2017). Adaptive Mutations in RNA Polymerase and the Transcriptional Terminator Rho Have Similar Effects on Escherichia coli Gene Expression.. Molecular biology and evolution, 34(11), 2839-2855. doi: 10.1093/molbev/msx216. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/40j8s0dm Molecular Biology and Evolution, 34 (11) |
| ISSN: | 1537-1719 0737-4038 |
| Popis: | Modifications to transcriptional regulators play a major role in adaptation. Here, we compared the effects of multiple beneficial mutations within and between Escherichia coli rpoB, the gene encoding the RNA polymerase β subunit, and rho, which encodes a transcriptional terminator. These two genes have harbored adaptive mutations in numerous E. coli evolution experiments but particularly in our previous large-scale thermal stress experiment, where the two genes characterized alternative adaptive pathways. To compare the effects of beneficial mutations, we engineered four advantageous mutations into each of the two genes and measured their effects on fitness, growth, gene expression and transcriptional termination at 42.2 °C. Among the eight mutations, two rho mutations had no detectable effect on relative fitness, suggesting they were beneficial only in the context of epistatic interactions. The remaining six mutations had an average relative fitness benefit of ∼20%. The rpoB mutations affected the expression of ∼1,700 genes; rho mutations affected the expression of fewer genes but most (83%) were a subset of those altered by rpoB mutants. Across the eight mutants, relative fitness correlated with the degree to which a mutation restored gene expression back to the unstressed, 37.0 °C state. The beneficial mutations in the two genes did not have identical effects on fitness, growth or gene expression, but they caused parallel phenotypic effects on gene expression and genome-wide transcriptional termination. Molecular Biology and Evolution, 34 (11) ISSN:0737-4038 ISSN:1537-1719 |
| Databáze: | OpenAIRE |
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