Expression of PD-L1 on Immune Cells Shows Better Prognosis in Laryngeal, Oropharygeal, and Hypopharyngeal Cancer
| Autor: | Ede Birtalan, Violetta Piurkó, Zoltán Fent, Balazs Acs, Judit Halász, Bianka Gurbi, Biborka Antal, Gábor Polony, Kornél Dános, Reka Mihalyi, Diána Brauswetter, József Tímár, László Tamás, Anna Pató |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male Histology medicine.medical_treatment Lymphocyte B7-H1 Antigen Disease-Free Survival Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Immune system Lymphocytes Tumor-Infiltrating medicine Humans Laryngeal Neoplasms Papillomaviridae Aged Aged 80 and over Hypopharyngeal Neoplasms biology Tumor-infiltrating lymphocytes business.industry Hypopharyngeal cancer Immunotherapy Middle Aged medicine.disease Primary tumor Neoplasm Proteins Gene Expression Regulation Neoplastic Survival Rate Medical Laboratory Technology Oropharyngeal Neoplasms 030104 developmental biology medicine.anatomical_structure CTLA-4 030220 oncology & carcinogenesis Cancer research biology.protein Carcinoma Squamous Cell Female Antibody business |
| Zdroj: | Applied immunohistochemistrymolecular morphology : AIMM. 26(7) |
| ISSN: | 1533-4058 |
| Popis: | Despite great enthusiasm towards immunotherapy, reliable biomarkers are still lacking. The importance of subsets based on human papillomavirus (HPV) status is supported by a growing body of evidence. However, role of other possible subgroups such as anatomic localization of primary tumor remains controversial. Our objective was to investigate immune cell infiltrate and checkpoint inhibitor proteins in above-mentioned head and neck cancer subsets. Archival tumor samples of 106 laryngeal, oropharyngeal, and hypopharyngeal cancer patients were stained with PD-L1, PD-L2, PD-1, and CTLA-4 antibodies. Proportion of tumor-infiltrating lymphocytes was assessed as well. In HPV-negative tumors, PD-L1 immune cell positivity was associated with better disease-specific survival. PD-L1 expression on immune cells correlated with improved disease-specific survival in laryngeal tumors. Furthermore, PD-L1 immune cell positivity correlated with CTLA-4 expression on immune cells and it was accompanied by high tumor-infiltrating lymphocyte rate. PD-L1 expression on tumor cells and PD-1 status showed strong correlation in all patients and in oropharyngeal and laryngeal localization, but not in hypopharynx. HPV-negative oropharyngeal cancers showed negative PD-L1 status on tumor cells. CTLA-4 positivity was observed in 49.5% and 20.6% on immune cells and on tumor cells, respectively. We concluded that PD-L1 expression on immune cells indicates better prognosis in laryngeal squamous cell carcinoma and in HPV-negative head and neck squamous cell carcinoma. We have not found any essential differences between anatomic subgroups. A possibly distinct role of hypopharyngeal localization regarding immune activity requires further clarification. |
| Databáze: | OpenAIRE |
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