Upregulation of Mir342 in Diet-Induced Obesity Mouse and the Hypothalamic Appetite Control
| Autor: | Satoshi Yamaguchi, Atsunori Kamiya, Jun Eguchi, Ryosuke Sugawara, Naoko Kurooka, Jun Wada, Dongxiao Zhang, Boxuan Yang, Atsuko Nakatsuka, Takeshi Y. Hiyama, Haya Hamed Hassan Albuayjan, Xinhao Zhang |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_specialty Endocrinology Diabetes and Metabolism Mice Obese Adipose tissue Type 2 diabetes Biology Diet High-Fat Diseases of the endocrine glands. Clinical endocrinology abdominal obesity Mice Endocrinology Downregulation and upregulation Arcuate nucleus 3T3-L1 Cells Internal medicine microRNA medicine Transcriptional regulation Animals Humans Obesity hypothalamus Cells Cultured Original Research adipose tissues Mice Knockout Neurons Arcuate Nucleus of Hypothalamus Correction SNAP25 medicine.disease RC648-665 Up-Regulation Mice Inbred C57BL MicroRNAs Gene Expression Regulation Hypothalamus non-coding RNAs appetite regulation |
| Zdroj: | Frontiers in Endocrinology, Vol 12 (2021) Frontiers in Endocrinology |
| ISSN: | 1664-2392 |
| Popis: | In obesity and type 2 diabetes, numerous genes are differentially expressed, and microRNAs are involved in transcriptional regulation of target mRNAs, but miRNAs critically involved in the appetite control are not known. Here, we identified upregulation of miR-342-3p and its host gene Evl in brain and adipose tissues in C57BL/6 mice fed with high fat-high sucrose (HFHS) chow by RNA sequencing. Mir342 (-/-) mice fed with HFHS chow were protected from obesity and diabetes. The hypothalamic arcuate nucleus neurons co-express Mir342 and EVL. The percentage of activated NPY+pSTAT3+ neurons were reduced, while POMC+pSTAT3+ neurons increased in Mir342 (-/-) mice, and they demonstrated the reduction of food intake and amelioration of metabolic phenotypes. Snap25 was identified as a major target gene of miR-342-3p and the reduced expression of Snap25 may link to functional impairment hypothalamic neurons and excess of food intake. The inhibition of miR-342-3p may be a potential candidate for miRNA-based therapy. |
| Databáze: | OpenAIRE |
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