A novel sugar-probe biosensor for the deadly plant proteinous toxin, ricin
| Autor: | Hirotaka Uzawa, Yukiko Shinozaki, Takehiro Nagatsuka, Koji Ohga, Isaac Ohsawa, Yasuo Seto, Yoshihiro Nishida |
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| Rok vydání: | 2008 |
| Předmět: |
White powder
Biomedical Engineering Biophysics Carbohydrates Biosensing Techniques Ricin medicine.disease_cause Sensitivity and Specificity chemistry.chemical_compound Electrochemistry medicine Surface plasmon resonance Sugar biology Toxin Chemistry Ricinus Reproducibility of Results General Medicine Equipment Design Surface Plasmon Resonance biology.organism_classification Equipment Failure Analysis Biochemistry Nanoparticles Gold Biosensor Biotechnology Visual monitoring |
| Zdroj: | Biosensorsbioelectronics. 24(4) |
| ISSN: | 1873-4235 |
| Popis: | Because of the illegal use of highly toxic ricin from the castor-oil plant, Ricinus communis, in bioterrorism and suspected white powder cases, anti-terrorism measures for the toxin are urgently required. Here we demonstrate a facile and sensitive detection method using synthetic analogues of β-lactosyl- and β- d -galactosyl ceramides as the ligands based on the fact that ricin binds cell-surface oligosaccharides. Sugar-probes having lipoic acids as anchor functions were synthesized via either a chemical or chemoenzymatic way and were immobilized on the sensor chips by a self-assembled monolayer technique. Surface plasmon resonance (SPR) analysis using these carbohydrate probes allowed us to detect the toxin in a highly sensitive and facile manner (10 pg/mL, 5 min), being the best benchmark as a method for detecting the toxin. In addition, a visual monitoring method was developed, in which sugar-coated Au nanoparticles were utilized for discriminating ricin from other proteins in a facile manner, taking 10–30 min for judgment. |
| Databáze: | OpenAIRE |
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