Biomedical nanoparticles modulate specific CD4+ T cell stimulation by inhibition of antigen processing in dendritic cells

Autor:	P Gerber, Usawadee Sakulkhu, Karin De Peyer, Barbara Rothen-Rutishauser, Christophe von Garnier, Heinrich Hofmann, Alke Petri-Fink, Thomas Geiser, Jatuporn Salaklang, Laurent P. Nicod, Fabian Blank, Peter Gehr
Rok vydání:	2011
Předmět:	CD4-Positive T-Lymphocytes Lipopolysaccharides Cytochalasin D dendritic cell T cell Antigen presentation Biomedical Engineering Apoptosis 02 engineering and technology Biology Toxicology Ferric Compounds immune response 03 medical and health sciences Immune system Antigen medicine antigen processing Humans Magnetite Nanoparticles Cells Cultured 030304 developmental biology CD86 0303 health sciences HLA-D Antigens Antigen processing Dendritic cell Dendritic Cells 021001 nanoscience & nanotechnology Flow Cytometry Molecular biology Cell biology antigen presentation medicine.anatomical_structure SPIONs 0210 nano-technology CD80
Zdroj:	Nanotoxicology
DOI:	10.3109/17435390.2010.541293
Popis:	Understanding how nanoparticles may affect immune responses is an essential prerequisite to developing novel clinical applications. To investigate nanoparticle dependent outcomes on immune responses dendritic cells (DCs) were treated with model biomedical poly(vinylalcohol) coated super paramagnetic iron oxide nanoparticles (PVA SPIONs). PVA SPIONs uptake by human monocyte derived DCs (MDDCs) was analyzed by flow cytometry (FACS) and advanced imaging techniques. Viability activation function and stimulatory capacity of MDDCs were assessed by FACS and an in vitro CD4 + T cell assay. PVA SPION uptake was dose dependent decreased by lipopolysaccharide (LPS) induced MDDC maturation at higher particle concentrations and was inhibited by cytochalasin D pre treatment. PVA SPIONs did not alter surface marker expression (CD80 CD83 CD86 myeloid/plasmacytoid DC markers) or antigen uptake but decreased the capacity of MDDCs to process antigen stimulate CD4 + T cells and induce cytokines. The decreased antigen processing and CD4 + T cell stimulation capability of MDDCs following PVA SPION treatment suggests that MDDCs may revert to a more functionally immature state following particle exposure. {çopyright} 2011 Informa UK Ltd.
Databáze:	OpenAIRE
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