| Autor: |
M. Vahed, F. Ramezani, V. Tafakori, V. S. Mirbagheri, A. Najafi, G. Ahmadian |
| Rok vydání: |
2020 |
| Předmět: |
|
| DOI: |
10.6084/m9.figshare.12916615 |
| Popis: |
Additional file 1: Figure S1. Schematic diagram Of Protein A domains. Figure S2. A) DNA Sequence of p Lpp’-ompA-Spa construct cloned in pET26b, and B) protein sequence of p Lpp’-ompA-Spa construct. Figure S3. Schematic diagram showing the strategy for making recombinant plasmid pLpp’-ompA-Spa. Figure S4. Investigation of electrophoretic mobility shift assay of different recombinant clones on 1% agarose gel to confirm cloning using quick check extraction method. Figure S5. Investigation of enzymatic cleavage of recombinant pLpp’-ompA plasmids with NdeI and EcoRI restriction enzymes by 1% agarose gel electrophoresis. Figure S6. Amplification of protein A using PCR reactions at different temperatures from 54 to 57 from left to right. Figure S7. Enzymatic digestion of non-recombinant and recombinant plasmids pET26. Figure S8. Investigation of electrophoretic mobility shift assay of different recombinant clones on 1% agarose gel to confirm cloning using quick check extraction method. Figure S9. Relative expression and purification of Lpp’-ompA and Lpp’-ompA-Spa in E. coli (BL21-DE3) under control of the T7 promoter. Figure S10. Expression of Lpp’-ompA-Spa construct in E. coli (BL21-DE3). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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