Highly Protective Association of MMP-2-1306C/T Promoter Polymorphism With Asthma in a North Indian Population: A Pilot Study
| Autor: | Niti Birbian, Jagtar Singh, Surinder K. Jindal |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Pulmonary and Respiratory Medicine
Genetics medicine.medical_specialty matrix metalloproteinase-2 (MMP-2) business.industry Immunology Single-nucleotide polymorphism medicine.disease Asthma polymorphism Pathogenesis collagenase Molecular genetics Genetic variation Genotype medicine Immunology and Allergy SNP Original Article Allele business |
| Zdroj: | Allergy, Asthma & Immunology Research |
| ISSN: | 2092-7355 |
| Popis: | PURPOSE Asthma is the most prevalent disease in India according to the national survey conducted by NFHS 2 in 1998-1999. Matrix metalloproteinase-2 (MMP-2), a collagenase encoded by the MMP-2 gene, degrades the type IV collagen and is responsible for inflammatory responses. This is a pilot study evaluating the role of MMP-2 -1306C/T promoter single nucleotide polymorphism (SNP) in asthma pathogenesis. METHODS A case-control study was performed with a total of 824 adult subjects, including 410 adult asthmatics and 414 healthy controls from regions of North India. The MMP-2 -1306C/T polymorphism was genotyped by the Tetra-Primer Amplification Refractory Mutation System Polymerase Chain Reaction (Tetra-Primer ARMS PCR). RESULTS Statistical analysis of the results for the MMP-2 -1306C/T polymorphism revealed an extremely protective role of the mutant T allele in asthma pathogenesis with OR=0.45, 95% CI (0.35-0.58) and P=0.000. The heterozygous CT genotype also conferred protection from asthma with OR=0.37, 95% CI (0.27-0.51) and P=0.000. The homozygous TT genotype was also significantly associated with asthma with OR=0.35, 95% CI (0.16-0.72) and P=0.002. Moreover, the polymorphism was significantly associated with all the phenotypic traits of the disease. CONCLUSION The MMP-2 -1306C/T promoter polymorphism confers significant protection from asthma in the studied North Indian population. |
| Databáze: | OpenAIRE |
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